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Cd8 negative selection

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The CD8 negative selection product is a laboratory tool used to isolate specific cell types from a mixed cell population. It allows for the selective removal of CD8+ cells, resulting in a purified sample that is enriched for the remaining cell types.

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Lab products found in correlation

Celltrace violet, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Negative selection (51 citations) CD8 negative selection kit (10 citations) Negative selection CD8 T cell isolation kit (3 citations) CD8-negative selection MACS column (2 citations) CD8+ T-cell negative selection kit (2 citations) Human CD8 negative selection kit (2 citations) CD8 magnetic bead negative selection kit (2 citations) MACS CD8+ T cell negative selection kit (2 citations)

5 protocols using cd8 negative selection

1

Adoptive Transfer of Naive CD8+ T Cells

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For adoptive transfer of naive cells, CD8+ T cells were isolated from spleens of naive Batf−/− or wild-type P14 mice by CD8 negative selection (Miltenyi Biotec). The indicated number (1 × 103–106) of 1:1 mixed, or single P14 cells, were transferred into nonirradiated naive recipient mice. For proliferation assays, P14 cells were labeled with 10μM CFSE or Cell Trace Violet (Invitrogen) before transfer. For flow cytometry analysis after infection, major lymphoid and non-lymphoid organs were removed on the days indicated, and single cell suspensions were prepared as previously described39 (link). RBCs in the cell suspensions were lysed using ammonium chloride. To transfer memory P14 cells, Batf−/− and wild-type memory P14 cells were generated in the two groups of mice. Spleen cells containing P14 cells were isolated, mixed and transferred into new recipient mice.
Kurachi M., Barnitz R.A., Yosef N., Odorizzi P.M., Dilorio M.A., Lemieux M.E., Yates K., Godec J., Klatt M.G., Regev A., Wherry E.J, & Haining W.N. (2014). The transcription factor BATF operates as an essential differentiation checkpoint in early effector CD8+ T cells. Nature immunology, 15(4), 373-383.
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2

Adoptive Transfer of Naive CD8+ T Cells

Cited 1 time
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For adoptive transfer of naive cells, CD8+ T cells were isolated from spleens of naive Batf−/− or wild-type P14 mice by CD8 negative selection (Miltenyi Biotec). The indicated number (1 × 103–106) of 1:1 mixed, or single P14 cells, were transferred into nonirradiated naive recipient mice. For proliferation assays, P14 cells were labeled with 10μM CFSE or Cell Trace Violet (Invitrogen) before transfer. For flow cytometry analysis after infection, major lymphoid and non-lymphoid organs were removed on the days indicated, and single cell suspensions were prepared as previously described39 (link). RBCs in the cell suspensions were lysed using ammonium chloride. To transfer memory P14 cells, Batf−/− and wild-type memory P14 cells were generated in the two groups of mice. Spleen cells containing P14 cells were isolated, mixed and transferred into new recipient mice.
Kurachi M., Barnitz R.A., Yosef N., Odorizzi P.M., Dilorio M.A., Lemieux M.E., Yates K., Godec J., Klatt M.G., Regev A., Wherry E.J, & Haining W.N. (2014). The transcription factor BATF operates as an essential differentiation checkpoint in early effector CD8+ T cells. Nature immunology, 15(4), 373-383.
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3

Purification and Adoptive Transfer of TCR Transgenic T Cells

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Cell suspensions were generated from spleens and lymph nodes from congenic 2C/Rag2−/−/CD45.1/2 and/or P14/Rag2−/−/CD45.2 mice, and T cells were purified by CD8+ negative selection (Miltenyi Biotec) over magnetic columns according to the manufacturer’s protocol. TCR Tg T cells were washed with PBS and resuspended at a concentration of 10 × 106/ml, and 106 TCR Tg cells were adoptively transferred into CD45.1 tumor bearing mice by tail vein transfer in a volume of 0.1 ml. After the indicated times, 2C T cells and corresponding host CD8+ T cells were sorted for in vitro stimulation.
Williams J.B., Horton B.L., Zheng Y., Duan Y., Powell J.D, & Gajewski T.F. (2017). The EGR2 targets LAG-3 and 4-1BB describe and regulate dysfunctional antigen-specific CD8+ T cells in the tumor microenvironment. The Journal of Experimental Medicine, 214(2), 381-400.
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4

LCMV Infection of Adoptively Transferred CD8+ T Cells

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CD8+ T cells were isolated from the spleen of P14 (CD45.1+) and P14ΔGpx4/ΔGpx4 (CD45.2+) by CD8 negative selection (Miltenyi Biotec). Cells mixed at equal ratio (1:1 ratio; 106 cells) were transferred into naive recipient mice (CD45.1+CD45.2+). The recipient mice were infected with LCMV-WE (1,000 pfu) 2 h after transfer, and splenocytes were analyzed at day 3 and 4 after transfer. P14 cells were distinguished by Vα2+CD8+ cells.
Matsushita M., Freigang S., Schneider C., Conrad M., Bornkamm G.W, & Kopf M. (2015). T cell lipid peroxidation induces ferroptosis and prevents immunity to infection. The Journal of Experimental Medicine, 212(4), 555-568.
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5

Adoptive Transfer of CD8+ T Cells

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For adoptive transfer experiment, naïve CD8+ T cells were isolated from spleens and lymph of naïve Cul4bcKO or wild-type P14 mice by CD8 negative selection (Miltenyi Biotec). The indicated number (5 × 104–105) of 1:1 mixed, or single P14 CD8+ T cells, were transferred by intravenous injection into nonirradiated naïve recipient mice (B6.CD45.1). For flow cytometry analysis after infection, major lymphoid organs were removed on the days indicated, and single cell suspensions were prepared as previously described25 (link). RBCs in the cell suspensions were lysed using ammonium chloride.
Dar A.A., Kim D.D., Gordon S.M., Klinzing K., Rosen S., Guha I., Porter N., Ortega Y., Forsyth K.S., Roof J., Fazelinia H., Spruce L.A., Eisenlohr L.C., Behrens E.M, & Oliver P.M. (2023). c-Myc uses Cul4b to preserve genome integrity and promote antiviral CD8+ T cell immunity. Nature Communications, 14, 7098.
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