After approximately one-year post SHIV challenge, the trispecific antibody treatment animals were injected intravenously with anti-CD8β mAb CD8b255R1 (National Institutes of Health Nonhuman Primate Reagent Resource Program) (50mg/kg). Whole blood was obtained and stained with fluorescently conjugated antibodies at different time points before and after infusion to monitor absolute counts of lymphocyte subsets in the blood using fluorescent bead containing trucount tubes (BD biosciences) as previously described (Pegu et al., 2015 (link)). In brief, the following antibodies were used to monitor the various lymphocyte subsets – anti-NHP CD45 FITC (clone D058-1283, BD Biosciences), anti-CD20 Pacific Blue (clone 2H7, BioLegend), anti-CD14 BV510 (M5E2, BioLegend), anti-CD69 BV605 (clone FN50, BioLegend), anti-CD16 BV711 (clone 3G8, BioLegend), anti-CD8 BV785 (clone RPA-T8, BioLegend), anti-CD4 Alexa Flour 700 (clone OKT4, BioLegend), anti-CD3 APC-Cy7 (clone SP34-2, BD Biosciences), anti-HLA-DR PE-Cy5.5 (clone TU36, ThermoFisher) and anti-CD159a PE-Cy7 (clone REA110, Milenyi Biotec). In addition, plasma viremia was quantified for up to 6 months post CD8+ T cell depletion.
Anti hla dr pe cy5.5 clone tu36
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-HLA-DR PE-Cy5.5 (clone TU36) is a fluorescently labeled antibody used for the detection and analysis of HLA-DR expressing cells in flow cytometry applications. It binds to the HLA-DR antigen, which is expressed on the surface of antigen-presenting cells, such as B cells, monocytes, and dendritic cells. The PE-Cy5.5 fluorescent dye is attached to the antibody, allowing for its detection and quantification using flow cytometry instrumentation.
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Lab products found in correlation
2 protocols using anti hla dr pe cy5.5 clone tu36
1
CD8+ T Cell Depletion Monitoring
2
Phenotypic Analysis of PBMCs
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When samples were available, PBMCs were stained with zombie UV dye (Biolegend, USA) and surface stained with Lineage cocktail 3-FITC, anti-b7-BV605 (clone FIB504), anti-CD141-BV650 (clone 1A4), anti-CD103-BV711 (BerACT8), anti-CD83-BUV395 (clone HB15e), anti-CD16-BUV496 (clone 3G8), anti-CD56-BUV563 (NCAM16.2), anti-CD11c-BUV661 (clone B-ly6), anti-CD86-BUV737 (clone 2331), anti-CD4-BUV805 (clone SK3), anti-CCR7-Ax700 (clone 150502), anti-CCR5-APCCy7 (clone 2D7/CCR5), anti-CD40-PECy5 (clone 5C3), and anti-PDL1-PECy7 (clone MIH1) (BD Bioscience, USA), anti-CD123-BV421 (clone GH6), anti-CD1c-BV510 (clone LI61), anti-BDCA2-BV785 (clone 201A), anti-CCR2-APC (clone K036C2), and anti-CCR9-PE (clone L053E8) (Biolegend, USA), anti-CD2-PETexaRed (clone RPA-2.10), and anti-HLADR-PECy5.5 (clone TU36) (Thermo Fisher, USA) for 20 min at room temperature and then washed twice and fixed in PBS containing 4% PFA. Cells were acquired on a 30-parameters A5 Symphony flow cytometer using FACS Diva Software (BD Bioscience, Bethesda, USA); data were analyzed using the FlowJo software (Treestar, Ashland, OR).
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