Mitotic metaphase chromosomes were prepared by using the root tips according to the procedure described by She et al. (2006) (link). The actively growing root tips were collected from potted plants and treated with saturated α-bromonaphthalene for 1.5 h at 28 °C, fixed in 3:1 (v/v) methanol/glacial acetic acid for at least 12 h at room temperature, and then stored at 4 °C until use. The fixed root tips were then washed in double distilled water and citrate buffer (0.01 mM citric acid-sodium citrate, pH 4.5) for 10 min each and incubated in a mixture of 2% cellulase R-10 (Yakult Pharmaceutical Industry, Tokyo, Japan), 2% pectolyase Y-23 (Yakult Pharmaceuticals), and 2% macerozyme R-10 (Sigma-Aldrich, Steinhem, Germany) in citric acid buffer at 28 °C for 2.5~3 h. Root tips were transferred to a glass slide along with the fixative and dissected using fine-pointed forceps. Finally, the slides were dried above a flame. Good preparations were selected by Olympus BX60 phase contrast microscope, and then stored at -20 °C.
Bx60 phase contrast microscope
Manufactured by Olympus
Sourced in Japan
The Olympus BX60 phase contrast microscope is a high-quality optical instrument designed for professional laboratory applications. It provides clear and detailed observation of samples by utilizing phase contrast technology. The BX60 microscope is capable of generating high-contrast images without the need for staining or special sample preparation.
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Lab products found in correlation
2 protocols using bx60 phase contrast microscope
1
Preparation of Mitotic Chromosomes from Plant Root Tips
2
Mixed-species Biofilm Formation on Cover Glass
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Mixed-species biofilms were formed on a cover glass by culturing at 30°C for 6 hr in 2 mL of YPD medium in a petri dish. After 6
hr cultivation, the cover glasses were washed twice and dried at room temperature for 30 min (some samples were Gram-stained),
observed with the Olympus BX60 phase contrast microscope equipped with a UPlanFl oil immersion lens (Olympus, Tokyo) and
photographed with a DP90 digital camera (Olympus).
hr cultivation, the cover glasses were washed twice and dried at room temperature for 30 min (some samples were Gram-stained),
observed with the Olympus BX60 phase contrast microscope equipped with a UPlanFl oil immersion lens (Olympus, Tokyo) and
photographed with a DP90 digital camera (Olympus).
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