Metabolite standards

Progesterone injection was purchased from Xianju Pharma (Taizhou, China). Pentobarbital was obtained from Xiya Reagent (Chengdu, China). Ultrapure water was produced by a Milli-Q plus water purification system (Milford, USA). N,O-bis(trimethylsilyl)-trifluoroacetamide-trimethylchlorosilane (BSTFA-TMCS) (99 : 1, v/v), urease, margaric acid, tropic acid, methoxylamine hydrochloride, pyridine, and metabolite standards were purchased from Sigma-Aldrich (St. Louis, USA). Absorbable gelatin sponge was purchased from Jinling Pharmaceutical Co. (Nanjing, China).
PSF was purchased from Tianxiang Co. (Yueyang, China) and identified by Professor Zhuxin Wang (Hunan university of Chinese medicine, Changsha, China). A voucher specimen (number HHBJC20140913) is deposited in the Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics & Gynecology Research (the Maternal and Child Health Hospital of Hunan Province, Changsha, China). Referring to previous method [25 (link)], a 500 g of PSF was two times extracted with 5 L of hot ethanol for 1 hour and then the two extracts were filtrated. The filtrate was dried in vacuo to yield 63.9 g of the PSF extract.

Acetonitrile (ACN), methanol (MeOH), and water were of LC‐MS grade and ordered at Fisher Scientific (Vienna, Austria) or Sigma‐Aldrich (Vienna, Austria). ABC, ammonium formate, and ammonium hydroxide were purchased as the eluent additive for LC‐MS at Sigma‐Aldrich. Formic acid was also of LC‐MS grade and ordered at VWR International (Vienna, Austria). Sodium hydroxide (NaOH) was obtained from Sigma‐Aldrich (Vienna, Austria). Metabolite standards were purchased from Sigma‐Aldrich (Vienna, Austria) or Carbosynth (Berkshire, UK).

Metabolite standards, 3 N methanolic HCl, and toluene were purchased from Sigma. [U-¹³C]Glucose, [U-¹³C]glycine, and [U-¹³C]fumarate were obtained from Isotec. Potassium hydroxide (KOH), methylene chloride, ethoxyamine hydrochloride, and MSTFA+1% TMCS (N-methyl-N-trimethylsilytrifluoroacetamide plus 1% trimethylchlorosilane), solvents for GC-MS and LC-MS/MS, were purchased from Fisher Scientific. Gibberellins (GA4/GA7) and Murashige and Skoog basal salt were ordered from PhytoTechnology Laboratories.

Optima LC/MS grade acetonitrile and water were purchased from Thermo Fisher Scientific Inc. (Pittsburgh, PA, USA). Metabolite standards were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cef was obtained from the MP Biomedicals (Santa Ana, CA, USA).

Optima LC grade acetonitrile was purchased from Merck (Merck, Darmstadt, Germany), formic acid was purchased from Fluka (Honeywell, Morris Plains, NJ, United States), and ultrapure water was prepared using a Milli-Q purification system (Merck Millipore, Darmstadt, Germany). Primary antibodies were purchased from Abcam (Cambridge, MA, United States): anti-ERα (ab3575), anti-ERβ (ab3576), anti-Ggt1 (ab109427), and anti-Anpep (CD13; ab108310). Metabolite standards were obtained from Sigma-Aldrich (Spruce St., St Louis, MO, United States).