Harvested plants were first rinsed with 5 mM CaCl2 for 5 min and then rinsed three times with ultrapure water. Afterward, the samples were dried in an oven at 65°C for 72 h, and the dried weight was recorded. The plant samples were then digested using HNO3 at 140°C until the mixture became clear. After dilution with ultrapure water and filtering with a 0.22-μm filter, the Fe and other element concentrations were analyzed by a microwave plasma-atomic emission spectroscope (MP-AES, Agilent Technologies, Santa Clara, CA, United States).
Mp aes
Manufactured by Agilent Technologies
Sourced in United States, Australia
The MP-AES (Microwave Plasma-Atomic Emission Spectroscopy) is a laboratory instrument designed for the analysis of elemental composition in various samples. It utilizes a microwave-induced plasma to atomize and excite the sample, enabling the detection and quantification of multiple elements simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Cyclone plus, by PerkinElmer (2 mentions)
Suprapur 14 mol l hno3, by Merck Group (2 mentions)
Milli q water, by Merck Group (2 mentions)
Vario el 3, by Elementar (1 mentions)
Db wax capillary column, by Agilent Technologies (1 mentions)
Agilent 6890n, by Agilent Technologies (1 mentions)
Vario micro cube, by Elementar (1 mentions)
Filter paper, by Cytiva (1 mentions)
D5000, by Siemens (1 mentions)
Supra 55vp, by Zeiss (1 mentions)
Multi element standard analytical solutions, by Merck Group (1 mentions)
22 protocols using mp aes
1
Plant Elemental Analysis Protocol
2
Ionome Analysis of Dried Shoot Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
From three to five replicates per accession were pooled and used for each ionome analysis. Total C and N content was obtained from dried shoot tissue using an Elementar Pyrocube analyzer. Cu, Fe, Mg, Mn, Na, and Zn content was obtained from dry shoot tissue mixed with 750 µl of nitric acid (65% [v/v]) and 250 µl of hydrogen peroxide (30% [v/v]). After one night at room temperature, samples were mineralized at 85 °C during 24 hr. Once mineralized, 4 ml of milliQ water was added to each sample. Mineral contents present in the samples were then measured by microwave plasma atomic emission spectroscopy (MP-AES, Agilent Technologies).
3
Biosynthesis and Characterization of Gold Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A stock solution of 10.0 mg/mL of the CM extract in 50% aqueous ethanol was prepared for the synthesis of AuNPs. The AuNPs were prepared as previously described [41 ]. Briefly, 2 mL of CM extract was added to 5 mL of 1 mM aqueous chloroauric acid (HAuCl4·3H2O) and the mixture was allowed to incubate at room temperature. The formation of AuNPs was indicated by the change of color of the suspension from yellow to red-violet. The AuNPs produced were then purified by repeated centrifugation at 14,000 rpm for 30 min at 4 °C, after which AuNP pellets were suspended in ultrapure water. The microwave plasma-atomic emission spectroscopy (MP-AES) (Agilent Technologies, Santa Cara, CA, USA) technique was utilized to determine the final concentration of AuNPs in the suspension.
4
Comprehensive Biomethanation Substrate Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The parameters i.e. SCOD, COD, alkalinity, TS, and VSS were analyzed according to Federation, 2005 . The metal content of substrates was determined using the Microwave Plasma Atomic Emission Spectrometer (MP-AES, Agilent 4100, America). Chinese standard methods (Chinese National Standard, 2010a ,b ) were used to measuring Protein, carbohydrates, lipids, cellulose, and calcium of substrates. 2 mL of sample was enough to analyze the VFAs and the pH of the solution. 0.1 mL of the extracted sample was injected into GC (Agilent 6890 N, USA) equipped with a flame ionization detector, DB-wax capillary column (Agilent Technologies, USA), and helium gas as a carrier (flow rate of 4 mL min − 1) to determining the characteristics of VFAs (Bardi and Rad. 2019). The pH of samples was measured using a pH meter (PCE-PHD 1). An element analyzer (Vario EL III, Elementar, Germany) was used to determine the ratio of C, and N in the organic substrates. The content of CO2 and CH4 in biogas was determined by gas chromatograph (GC) (Agilent Technologies 6890 N, USA). All samples were analyzed in triplicate.
5
Microwave-assisted Mineral Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
About 15 mg (DW: dry weight) of ground sample (rosette leaves or roots) were mixed with 750 μl nitric oxide (65%) and 250 μl hydrogen peroxide 30% before homogenization. Samples were left over night at room temperature and then mineralized 7h at 84°C. Once mineralized, the nitric oxide proportion present in the samples was adjusted to 5 to 10% of the final volume by adding ultrapure water. Minerals content present in the samples was then measured by microwave plasma atomic emission spectroscopy (MP-AES, Agilent).
6
Quantifying Mn-52 Chelation Efficiency
7
Characterization of Magnetic Microspheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Magnetic properties of the microspheres were determined by magnetic susceptibility measurements (MS2G, Bartington, Witney, Oxfordshire, UK). At first, 1 mg of dried microspheres was placed in the measuring tube. Then the tube was positioned in the column or aperture with the magnetic susceptibility sensors and systems in order to obtain the data. For the iron content determination, the microspheres were dispensed in 65% HNO3 and diluted with deionized water (1:9) prior to the detection of iron in the microspheres via microwave plasma atomic emission spectroscopy (MP-AES, Agilent 4200).
8
Magnesium Oxide Slurry Purification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 1
11 g of 93% magnesium oxide was slurried with 70 g of water and 30 g pickling acid residue solution, containing 50% PAR with a pH of less than 0, was added to produce a final volume of 100 ml. The pH of this mix was 8.7. The solution was heated to 80° C. and the pH was adjusted to 6 with 15 g of 93% sulfuric acid. pH was continuously adjusted for 3 hours, after which the solution was filtered and analysed using a microwave plasma-atomic emission spectrometer (MP-AES, Agilent Technologies). The results are presented in Table 1. The residual material (FeNiCr-cake) was then dried and analysed, and used for further processing.
9
Metal Quantification in Intestinal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following intestinal epithelial cell isolations, cell pellets were digested at 80°C overnight in HNO3 to measure metal concentrations using microwave plasma-atomic emission spectrometry (MP-AES) (Agilent, Santa Clara, CA). Normalization was to total protein concentrations. Tissue metal concentrations were measured by MP-AES following overnight digestion at 80°C and appropriate dilutions for each tissue with deionized water. Normalization was to wet tissue weight. Blood was collected by cardiac puncture into EDTA tubes, and plasma was obtained by centrifugation at 3000 × g for 15 min. Following dilutions (1/5) in deionized water, plasma metal concentrations were measured by MP-AES.
10
Quantification of Calcium in Mussel Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The amount of calcium (ppm) in the mantle and culture medium was quantified using a Microwave Plasma-Atomic Emission Spectrometer (MP-AES, AGILENT). After incubation with CALCIIa peptide the mussel posterior mantle edge was washed in 1x PBS, dried at 60 °C for at least for 2 days and ground into a fine powder using a mortar and pestle. The tissue powder was weighed and digested at RT for 2 days in concentrated HNO3 (0.1 g of tissue/ml). The culture medium was also collected and centrifuged for 15 min, 12,000 rpm at RT to separate cells and tissue fragments and 20 µl of the clean supernatant was digested with 80 µl of concentrated HNO3. The reaction was performed for 2 days using the same conditions as for tissue digestion. All samples were subsequently kept at 4 °C and assayed within 4 days of hydrolysis. For MP-AES analysis tissues and medium were diluted 1:50 and 1:500, respectively with Normatom water (VWR, Portugal)/5% HNO3 to a final volume of 2 ml and calcium ion content determined at 393.366 nm. A standard curve for calcium was performed and used to establish calcium concentration.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!