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Mab 6c5 to gapdh

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MAb 6C5 to GAPDH is a mouse monoclonal antibody that specifically recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a ubiquitous enzyme involved in glycolysis and other cellular processes. This antibody can be used to detect and quantify GAPDH expression in various sample types using techniques such as western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Fluorescent licor irdye 800cw, by LI COR (1 mentions) Fluorescent licor irdye 700dx, by LI COR (1 mentions)

Close spelling variants of this product

GAPDH (6C5) GAPDH mAb GAPDH

2 protocols using mab 6c5 to gapdh

1

Quantification of α-synuclein phosphorylation

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Primary antibodies used were monoclonal antibody (mAb) Syn1 (BD Biosciences; 1:1000 in WB), mAb 6C5 to GAPDH (Santa Cruz; 1:1000 in WB), and polyclonal antibody (pAb) to αSyn pS129 EP1536Y (Abcam, 1:1000 in WB). Secondary antibodies used were LiCor IRDye 926–68,076, 926–32,212, and 926–32,211, 1:10,000 for WB. pS129/total αSyn Western blots were developed for total and pS129 αSyn on the same membrane (total αSyn in red channel, IRDye 926–32,212; pS129 αSyn in green channel, 926–32,211).
Nuber S., Chung C.Y., Tardiff D.F., Bechade P.A., McCaffery T.D., Shimanaka K., Choi J., Chang B., Raja W., Neves E., Burke C., Jiang X., Xu P., Khurana V., Dettmer U., Fanning S., Rhodes K.J., Selkoe D.J, & Scannevin R.H. (2022). A Brain-Penetrant Stearoyl-CoA Desaturase Inhibitor Reverses α-Synuclein Toxicity. Neurotherapeutics, 19(3), 1018-1036.
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2

Alpha-synuclein Protein Detection

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As primary antibodies we used mAb Syn1 to detect rat endogenous αS (Becton-Dickinson), mAb MJFR1 to detect only human αS (Abcam), mAb EP1537Y to βS (Abcam), mAb 6C5 to GAPDH (Santa Cruz), and pAb C4731 to Calnexin (Sigma). Secondary antibodies were anti-rabbit Fluorescent LiCor IRDye 800CW and anti-mouse Fluorescent LiCor IRDye 700DX.
Ramalingam N, & Dettmer U. (2021). Temperature is a key determinant of alpha- and beta-synuclein membrane interactions in neurons. The Journal of Biological Chemistry, 296, 100271.
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