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Transwell chambers with 8 μm pore

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Transwell chambers with 8-μm pores are a type of cell culture insert designed for use in cell migration and invasion assays. The chambers consist of a porous membrane with 8-micron diameter pores that separates an upper and lower compartment. Cells can be seeded in the upper chamber and their ability to migrate through the pores to the lower chamber can be measured as an indicator of their migratory or invasive potential.

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Lab products found in correlation

Matrigel, by BD (19 mentions) Matrigel, by Corning (5 mentions) Matrigel matrix, by BD (3 mentions) Ecm gel, by Merck Group (2 mentions) Transwell dish, by Corning (2 mentions) Phosphate buffered saline (pbs), by BD (2 mentions) Matrigel invasion chamber, by BD (2 mentions) Inverted microscope, by Olympus (2 mentions) Crystal violet, by Merck Group (2 mentions) Dmr inverted microscope, by Leica (1 mentions) 6 well cell culture plate, by Corning (1 mentions) Matrigel transwell chamber, by BD (1 mentions) Inverted light microscopy, by Leica (1 mentions) Crystal violet solution, by Beyotime (1 mentions) Paraformaldehyde (pfa), by Beyotime (1 mentions) Ckx3 slp, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Tnf α, by Novoprotein (1 mentions) Matrigel, by Merck Group (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Transwell cell culture chambers with 8-μm pores 6.5-mm Transwell chambers with 8-μm pores 8-μm Transwell chambers 8-μm chambers 8-μm pores Transwell chambers Pore transwells

47 protocols using transwell chambers with 8 μm pore

1

Wound Healing and Invasion Assay Protocol

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CCLP1 and HCCC-9810 were plated in each well of the 12-well plates and incubated to form 80–90% confluence. Then a scratch was performed with pipette tips. Fresh serum-free medium was changed. The wound closing procedure was observed for 24 h, and images were photographed.
Transwell chambers with 8 μm pores (Costar, Corning, NY, USA) were used to perform the invasion assay. Matrigel (BD Biosciences, New Jersey, USA) was coated on the top side of the insert membrane. The upper chamber was added with 200 μl serum-free medium and seeded 5 × 104 cells while the lower chamber was placed with 600 μl medium with 5% FBS. The chambers were maintained at 37 °C, 5% CO2 for 24 h. After that, the cells on the top side of the insert membrane were removed by cotton swabs. The inserts were then fixed in methanol for 20 min and stained with 1% crystal violet for 30 min. The cells on the bottom of the membrane were calculated under a microscope and photographed. All experiments were performed in triplicate.
Huang L., Jiang X., Li Z., Li J., Lin X., Hu Z, & Cui Y. (2020). Linc00473 potentiates cholangiocarcinoma progression by modulation of DDX5 expression via miR-506 regulation. Cancer Cell International, 20, 324.
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2

Cell Migration and Invasion Assay

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Transwell chambers with 8 μm pores (Costar; Corning, New York, NY, USA) were used to perform the migration and invasion assays. Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) was coated on the top side of the insert membrane in the invasion assay. The upper chamber was treated with 200 μL of serum-free medium and seeded with 104 cells, and the lower chamber was treated with 600 μL of medium with 5% FBS. The chambers were maintained at 37°C, 5% CO2 for 24 hours. Afterward, the unmigrated or uninvaded cells on the top of the insert membrane were removed by cotton swabs. The inserts were then fixed in methanol for 20 minutes and stained with 1% crystal violet for 30 minutes. The migrated or invaded cells on the bottom of the membrane were investigated under a microscope and photographed. All experiments were performed in triplicate.
Xia S., Wang C., Postma E.L., Yang Y., Ni X, & Zhan W. (2017). Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis. OncoTargets and therapy, 10, 1743-1755.
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3

Cell Proliferation and Migration Assay

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EdU (5-ethynyl-2’-deoxyuridine) staining was used to determine cell proliferation as described previously. As for cells migration assay, transwell chambers with 8-μm pores (Costar) were used following manufacturer’s instructions. Images were taken using a microscope (Leica Microsystems, Mannheim, Germany) at ×200 magnification.
Cai L., Chao G., Li W., Zhu J., Li F., Qi B., Wei Y., Chen S., Zhou G., Lu X., Xu J., Wu X., Fan G., Li J, & Liu S. (2020). Activated CD4+ T cells-derived exosomal miR-142-3p boosts post-ischemic ventricular remodeling by activating myofibroblast. Aging (Albany NY), 12(8), 7380-7396.
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4

Transwell Invasion Assay for Angiogenesis

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Transwell chambers with 8 μm pores (Costar, Corning, NY, USA) were used to perform invasion assays. Matrigel (BD Biosciences, NJ, USA) was coated on the top side of the inserts. Transwell invasion assay was performed on 4T1 cells and HUVECs. HUVECs were pretreated with supernatants of sublethal heat treated 4T1 cells culture. The upper chamber was filled with 200 μl serum-free medium, and 1×104 4T1 cells were seeded, while 600 μl medium with 5% FBS was added to the lower chamber. The chambers were maintained at 37° C in 5% CO2 for 24h. Then, cells on the upper chamber were removed by cotton swabs. The inserts were then fixed in 4% paraformaldehyde for 20 min and stained with 1% crystal violet for 30 min. The invaded cells on the bottom of the membrane were assessed using a microscope and photographed. All experiments were performed in triplicate.
Xia S., Li X., Xu S., Ni X., Zhan W, & Zhou W. (2022). Sublethal heat treatment promotes breast cancer metastasis and its molecular mechanism revealed by quantitative proteomic analysis. Aging (Albany NY), 14(3), 1389-1406.
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5

Transwell Invasion Assay for Lovo Cells

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The transwell chambers with 8 μm pores (Costar, Cambridge, MA, USA) were coated with 50 μL of a mixture of serum free DMEM and Matrigel (1:5 dilution, BD Biosciences, CA, USA), and then placed in a 24-well plate for 7 h at 37 °C. After solidification, the chambers were placed in the wells of a 24-well plate, including 600 μL of DMEM with 10% of serum, and the Lovo cells (1.5 × 105 cells) mixed with mIgG or R5 in serum free DMEM were added into the upper chambers. Following 20 h of incubation, the membranes of the chambers were fixed with methanol and stained with 1% crystal violet. Then, the cells on the upper membranes of the chambers were wiped, and the number of cells that had invaded the membranes was counted.
Yao Y., Zhou Z., Li L., Li J., Huang L., Li J., Qi C., Zheng L., Wang L, & Zhang Q.Q. (2019). Activation of Slit2/Robo1 Signaling Promotes Tumor Metastasis in Colorectal Carcinoma through Activation of the TGF-β/Smads Pathway. Cells, 8(6), 635.
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6

Cell Migration and Invasion Assay

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Transwell chambers with 8 μm pores (Costar, Corning, NY, USA) were used to perform migration and invasion assays. Matrigel (BD Biosciences, New Jersey, USA) was coated on the top of the insert membrane in the invasion assay. The upper chamber was filled with 200 μl serum-free medium, and 1×104 cells were seeded, while 600 μl medium with 5% FBS was added to the lower chamber. The chambers were maintained at 37°C in 5% CO2 for 24h. Then, the nonmigrated or noninvaded cells on the top side of the insert membrane were removed by cotton swabs. The inserts were then fixed in methanol for 20 min and stained with 1% crystal violet for 30 min. The migrated or invaded cells on the bottom of the membrane were assessed using a microscope and photographed. All experiments were performed in triplicate.
Xia S., Ji R., Xu Y., Ni X., Dong Y, & Zhan W. (2017). Twisted Gastrulation BMP Signaling Modulator 1 Regulates Papillary Thyroid Cancer Cell Motility and Proliferation. Journal of Cancer, 8(14), 2816-2827.
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7

Transwell Cell Migration and Invasion Assay

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Transwell chambers with 8-μm pores (Corning Costar, Corning, NY, USA) placed in 24-well plates were used to detect the ability of cells to migrate and invade.50% Chambers with or without Matrigel (BD, San Diego, CA, USA) was used to detect the cell invasiveness or migration. 600 μL of RPMI 1640 supplemented with 10% serum was added below the chamber, and 200 μL of serum-free RPMI 1640 containing cells was added above the chamber. After incubation for 24 h at 37°C in an atmosphere of 5% CO2, the chambers were placed into a 1.0% crystal violet. After 20 min, 1.0% crystal violet was washed with phosphate-buffered saline. Photographs were observed and taken with a microscope, and the number of cells passing through the chambers was counted with ImageJ.
Su H., Wang Y, & Li H. (2021). RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer. Frontiers in Genetics, 12, 768041.
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8

Cell Invasion Ability Assay

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Cell invasion abilities were performed with transwell assay using transwell chambers with 8-μm pores (Costar, Corning, NY, USA). In invasion assay, the upper side of the insert membrane was coated with matrigel (BD Biosciences, New Jersey, USA). A number of 1 × 104 cells in 200 μl of serum-free medium were added into the upper chamber and 600 μl of medium with 5% FBS as chemoattractant was added into the lower chamber. After 24 h’ incubation, the cells on the upper surface of the insert membrane were removed using cotton swab. The cells on the lower surface of the insert membrane were fixed in methanol for 20 min and then stained with 1% crystal violet for 30 min. The number of cells on the lower surface of the membrane was calculated with a microscope and then photographed. All experiments were performed in triplicate.
Xia S., Ji R, & Zhan W. (2017). Long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibits proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway. BMC Neurology, 17, 30.
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9

Transwell Migration Assay for HUVECs

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Transwell chambers with 8 μm pores (Costar, Cambridge, MA, USA) were applied to determine migration abilities. The upper chambers of transwell chambers were lled with HUVECs suspended in ECM medium while the lower chambers were lled with complete cell culture medium. The upper chambers were taken out after 24 hour incubation. After wiping the upper surfaces of the upper chambers and removing cells left on the upper surfaces, the bottom surfaces of these upper chambers were stained with 0.1% crystal violet to label migrated cells. Images were taken using a DMR inverted microscope (Leica Microsystems). Crystal violet was dissolved in 33% acetic acid and the optical densities (O.D.) of crystal violet were measured at 570 nm (Bio-Tek).
Chen S., zhang Y., Cai X., Yi S., & Wang X. (2021). miR-328a-3p stimulates endothelial cell migration and angiogenesis.
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10

Transwell Assay for Cell Migration and Invasion

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Transwell assays were used to investigate the migration and invasion abilities of the tumor cells. The cells were seeded on the upper Transwell chambers with 8-μm pores (Corning, USA) at a density of 2 × 104 cells per well with 200 µl of 2% FBS medium. Then, 800 μL of 15% FBS medium was added to the lower chamber of each Transwell chamber. Upper Transwell chambers with Matrigel were used for the invasion assays. After 48 h of incubation, each chamber was washed twice with PBS. Then, the cells that had invaded through the membranes were fixed using paraformaldehyde and were stained with crystal violet. Five random fields were photographed in each group, and the number of invaded cells were counted under a microscope.
Bo H., Zhu F., Liu Z., Deng Q., Liu G., Li R., Zhu W., Tan Y., Liu G., Fan J, & Fan L. (2021). Integrated analysis of high-throughput sequencing data reveals the key role of LINC00467 in the invasion and metastasis of testicular germ cell tumors. Cell Death Discovery, 7, 206.
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