Xlpln 25x wmp2

Manufactured by Zeiss

The XLPLN 25X WMP2 is a high-magnification objective lens designed for use in Zeiss microscopes. It provides a magnification of 25x and is optimized for wide-field observation. The lens is suitable for a variety of applications that require high-resolution imaging.

Automatically generated - may contain errors

Lab products found in correlation

Zen blue, by Zeiss (2 mentions) Fv1000mpe multi photon laser scanning microscope, by Zeiss (2 mentions) Plan apochromat 20x, by Zeiss (2 mentions) Premiere pro, by Adobe (1 mentions)

2 protocols using xlpln 25x wmp2

Imaging of GFP/RFP-labeled Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used an upright microscope set-up (Olympus FV1000MPE multi-photon laser scanning microscope or Zeiss 880, both with Spectra-Physics Mai Tai DeepSee 2-photon lasers) with water-immersion lens (Olympus XLPLN 25X WMP2 or Zeiss Plan-Apochromat 20X). The objective was immersed directly in the culture medium for imaging. The fluorophores used were all GFP or RFP derivatives, therefore the excitation wavelength was tuned between 925 and 935 nm. Laser power never exceeded 15%. Zen Blue (Zeiss) and ImageJ software was used to analyze movies. The Bleach Correction and Manual Tracking plugins were used to correct photobleaching and to track cells. The Correct 3D Drift plugin was used to correct for movements caused by tissue contraction. Adobe Photoshop (v21.1.3) and Adobe Premiere Pro (v14.2) were used to annotate and edit movies. Figures were generated using Adobe Illustrator (v24.1.3).

Bostock M.P., Prasad A.R., Chaouni R., Yuen A.C., Sousa-Nunes R., Amoyel M, & Fernandes V.M. (2020). An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. Frontiers in Cell and Developmental Biology, 8, 590094.

+ Open protocol

+ Expand

Intravital 2-Photon Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used an upright microscope set-up (Olympus FV1000MPE multi-photon laser scanning microscope or Zeiss 880, both with Spectra-Physics MaiTai DeepSee 2-photon lasers) with waterimmersion lens (Olympus XLPLN 25X WMP2 or Zeiss Plan-Apochromat 20X). The fluorophores used were all GFP or RFP derivatives, therefore the excitation wavelength was tuned between 925nm and 935 nm. Laser power never exceeded 15 %. Zen Blue (Zeiss) and ImageJ software was used to analyze movies. The Bleach Correction and Manual Tracking plugins were used to correct photobleaching and to track cells. The Correct 3D Drift plugin was used to correct for movements caused by tissue contraction. Adobe Photoshop (v21.1.3) and Adobe Premiere Pro (v14.2) were used to annotate and edit movies. Figures were generated using Adobe Illustrator (v24.1.3).

Bostock M.P., Prasad A.R., Chaouni R., Yuen A.C., Sousa‐Nunes R., Amoyel M., & Fernandes V.M. (2020). An immobilization technique for long-term time-lapse imaging of explantedDrosophilatissues.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!