Agilent 6820

The S. sanxanigenens strains shown in Table 1 were cultured in NK medium, and then collected when cultivated at 24 h. The crude total DNA-free RNA of S. sanxanigenens strains was extracted using the RNAiso Plus (Takara, Dalian, China) and RNAprep Pure Cell/Bacteria Kit (Tiangen, China). The cDNA was amplified using FastKing RT Kit (Tiangen, Beijing, China) with the total mRNA as the template. Samples were then analyzed using Agilent 6820 (Agilent, America) with RealMasterMix (SYBR Green I) (Tiangen, Beijing, China). Quantity real-time PCR amplification primers were listed in Table 2. For data analysis, the 16 S gene was selected as the internal standard for normalization between samples, and three biological replicates were performed. The obtained data were analyzed by using the 2^−ΔΔCt method [11 (link)].

The fatty acid composition of 49 vegetable oils (7 CAO brands, 7 RSO brands, 7 SBO brands, 7 SFO brands, 7 CO brands, 7 PO brands, and 7 SO brands) were measured as FAMEs using an Agilent 6820 gas chromatograph (Agilent, Shanghai, China) equipped with a flame ionization detector (FID). FAMEs were prepared according to a procedure reported previously [2 (link)].

The evaluation of photocatalytic performance was undergone in the quartz reactors. The LED lamp (5 W) was used as the light source. Specifically, H₅PMo₁₀V₂O₄₀/UiO-66-NH₂ composite (30 mg), acetonitrile (15 mL), acetic acid (3 mL) and benzene (1 mL) was added into the reactor, and gently stirred for a period of time. When the temperature of the solution was increased to 60 °C, the lamp was turned on. Different amounts of the H₂O₂ (5.65, 8.48, 11.30, 16.95, 22.60, 33.90 mmol) was titrated into the solution within 30 min. The reaction was maintained for 4 h. Then, the solution was treated by centrifugation. The qualitative and quantitative analysis of the supernatant was performed using toluene as internal standard by gas chromatography-mass spectrometry (GC-MS qp2010) and gas chromatography (Agilent 6820). In parallel research experiments, the best reaction conditions were explored by adjusting the ratio of H₂O₂ and benzene, reaction time and solvent.
The conversion of benzene, the selectivity of phenol and the yield of phenol are calculated as follows:

Phenylacetylene (2.0 mmol) was firstly absorbed by the Pd/GA (6 mg). The monolith was put into a 10 mL stainless steel autoclave, followed by evacuation and filling with H₂ for three times. Subsequently, H₂ with a suitable pressure was added to the autoclave, which was kept in a 30 °C water bath. After the desired time, the autoclave was depressurized slowly to ambient pressure. The Pd/GA was squeezed and washed with ethanol. The product was analyzed using gas chromatograph (Agilent 6820) equipped with a flame ionization detector (FID) and a PEG-20M capillary column (0.25 mm in diameter, 30 m in length).