Uplc esi ms ms

Manufactured by Waters Corporation

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The UPLC–ESI–MS/MS is an analytical instrument that combines Ultra-Performance Liquid Chromatography (UPLC), Electrospray Ionization (ESI), and Tandem Mass Spectrometry (MS/MS) technologies. This system is designed for the separation, identification, and quantification of a wide range of molecules in complex samples.

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Lab products found in correlation

Uplc beh c8 column, by Waters Corporation (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Atrazine, by J&K Scientific (1 mentions) Formic acid, by J&K Scientific (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) Cary eclipse, by Agilent Technologies (1 mentions) Cary 100 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Ammonium formate, by Merck Group (1 mentions) Phosphate buffer saline (pbs), by Merck Group (1 mentions) Masslynx 4, by Waters Corporation (1 mentions)

Close spelling variants of this product

UPLC-MS/MS (55 citations) UPLC-MS (45 citations) UPLC/ESI-MS) (3 citations) UPLC-ESI-MS/MS system (3 citations) NanoAcquity UPLC/ESI/MS (SYNAPT HDMS, (2 citations) Waters UPLC-ESI-Q/TOF-MS system (2 citations) UPLC/ESI-MS system (2 citations) NanoAcquityTM UPLC/ESI/q-TOF MS/MS (SYNAPTTM G2Si; (2 citations)

7 protocols using uplc esi ms ms

Quantification of ATZ and Chlorination Byproducts

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The concentration of ATZ and its oxidation products were detected using UPLC-ESI–MS/MS (Waters Corporation, Milford, MA, USA) equipped with an ACQUITY UPLC BEH C₈ column (2.1 mm × 100 mm, 1.7 μm particle). The detailed information of operation parameters is presented in Text S1 in the Supplementary materials. The extent of ATZ removal was calculated from Eq. (2).

A T Z r e m o v a l = \frac{{ATZ}_{0} - {ATZ}_{t}}{{ATZ}_{0}} \times 100

where ATZ₀ and ATZ_t were the detected ATZ concentrations at initial time and reaction time t, respectively.
The concentrations of DCAA and TCAA were measured with UPLC-ESI–MS/MS system based on our previous research^{22 (link)} with detailed information of operation parameters presented in Text S2 and Supplementary Table S1. The formations of TCP, TCM and CHP during chlorination process were detected by GC-QqQ-MS/MS (Agilent Technologies, Palo Alto, CA, USA) in the multiple reaction monitoring mode^{23 (link)} with specific operation parameters in Text S3 and Supplementary Table S2.

Liu Y., Zhu K., Zhu H., Zhao M., Huang L., Dong B, & Liu Q. (2020). Photooxidation of atrazine and its influence on disinfection byproducts formation during post-chlorination: effect of solution pH and mechanism. Scientific Reports, 10, 20355.

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Quantitative Analysis of Atrazine

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Atrazine was purchased from J&K Scientific (Beijing, China), with its purity ≥ 98.8%. Methanol and formic acid were of HPLC grade and were purchased from J&K Scientific (Beijing, China) and Kermel Chemical Reagent Co., Ltd. (Tianjin, China), respectively. A Milli-Q water purification system (MilliporeSigma, Burlington, MA, USA) was employed. Atrazine was prepared as a stock solution of 20 mg/L. The stock solution was kept in reserve at 4 °C until use.
Main testing instruments: Ultra-high-performance liquid-chromatography electrospray triple-quadrupole mass spectrometer (UPLC–ESI–MS/MS, Waters, Milford, MA, USA); an ACQUITYTM UPLC BEN C8 chromatographic column (2.1 mm × 50 mm, 1.7 μm, Waters, Milford, MA, USA).

Zhang Y., Zhao C., Yu A., Zhao W., Ren F, & Liu Y. (2022). The Migration Pattern of Atrazine during the Processes of Water Freezing and Thawing. Toxics, 10(10), 603.

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Multimodal Characterization of PFAS

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¹⁹F NMR data were recorded on a Bruker AV400 spectrometer. Steady-state fluorescence spectra were recorded in a conventional quartz cell (light path 10 mm) on a Cary Eclipse equipped with a Cary single-cell peltier accessory. UV–vis spectra were recorded in a quartz cuvette (light path 10 mm) on a Cary 100 UV–vis spectrophotometer equipped with a Cary dual cuvette peltier accessory. The sample solutions for dynamic light scattering measurements were examined on a laser light scattering spectrometer (NanoBrook 173plus) equipped with a digital correlator at 659 nm at a scattering angle of 90°. Quantification of PFOS and PFOA from the absorption studies were performed by means of UPLC–ESI–MS/MS (Waters, Milford, MA, USA).

Zheng Z., Yu H., Geng W.C., Hu X.Y., Wang Y.Y., Li Z., Wang Y, & Guo D.S. (2019). Guanidinocalix[5]arene for sensitive fluorescence detection and magnetic removal of perfluorinated pollutants. Nature Communications, 10, 5762.

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Malathion Detection by UPLC-ESI-MS/MS

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The main instruments were an ultra-performance liquid chromatography-electrospray ionization-triple quadrupole mass spectrometer (UPLC-ESI-MS/MS) (Waters, Milford, MA, USA) and ACQUITYTM UPLC BEN C8 column (2.1 mm × 50 mm × 1.7 μm). The instruments were used to detect malathion in samples. The experiment took place at the Micropollution Research Laboratory of Yantai University.
Main reagents: malathion from AccuStandard, purity ≥ 97.9%; methanol, from J&K, HPLC grade, purity ≥ 99.9%; and acetonitrile, from J&K, HPLC grade, purity ≥ 99.9%.

Zhang Y., Wang X., Zhao W., Liu Y., Liu T, & Yang P. (2023). The Migration Rules of Malathion during Indoor Simulated Lake Freezing. Toxics, 11(3), 222.

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Quantitation of Serum Sphingolipidome

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Ceramides, phosphorylated ceramides, sphingoid bases, phosphorylated sphingoid bases and sphingomyelins were extracted from serum (500 µL) using isopropanol:water:ethyl acetate (3:1:6; 4 mL per sample). Internal standards were added (50 pmol; Avanti Polar Lipids, Alabaster, AL, USA) and samples incubated on ice for 30 min. 3 mL of sample was dried, reconstituted in methanol with 0.1% formic acid, for analysis of ceramides, bases and phosphorylated species. The remaining 1 mL was extracted for sphingomyelins in 1:1:0.9 chloroform:methanol:water, the organic phase removed, dried, reconstituted in methanol (1 mL), alkaline hydrolysis performed and incubated for 2 h in darkness. The pH was neutralised, the sample dried and reconstituted in methanol with 0.1% formic acid. Samples were analysed using ultraperformance liquid chromatography with electrospray ionisation and tandem mass spectrometry (UPLC/ESI-MS/MS) (Waters, Wilmslow, UK) [20 (link)]. Analytes were identified by multiple reaction monitoring in the positive mode and relative quantitation performed using class-specific internal standards.

Krakowiak K., Maidstone R.J., Chakraborty A., Kendall A.C., Nicolaou A., Downton P., Cristian A.D., Singh D., Loudon A.S., Ray D.W, & Durrington H.J. (2023). Identification of diurnal rhythmic blood markers in bronchial asthma. ERJ Open Research, 9(4), 00161-2023.

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Quantification of Zearalenone by UPLC-ESI-MS/MS

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ZEA standard was purchased from Biopure (Tulln, Austria) and its stock solution (1000 µg/L) was prepared by dissolving it in acetonitrile. Other chemicals ammonium formate was obtained from Sigma Aldrich, formic acid was purchased from Fluka, phosphate buffer saline (PBS) was purchased from Sigma Aldrich (St. Louis, MO, USA). Buffer solutions were prepared in the range of pH 3-9 by using citric acid and disodium orthophosphate. pH measurement was done with pH meter ( Thermo orion model 420). Ultra-pure RO water (Milli Q) were utilised for all analysis Quantification of ZEA UPLC-ESI-MS/MS (Waters, Milford, MA, USA) was used for the quantification of ZEA. Separation was achieved on Acquity H-Class HSS T3 column (1.8μm 100mm x 2.1 mm,) with gradient mixture of mobile phase A (H2O/ HCOOH, 99/1 v/v/v, and 10 mM ammonium formate and mobile phase B (CH3OH/H2O/HCOOH, 97/2/1v/v/v, and 10 mM ammonium formate) at a flow rate of 0.5 mL/min as described [29] (link).

Sherazi S.T.H. (2020). Zearalenone Removal by Using Banana Peel as an Adsorbent. Pakistan Journal of Analytical & Environmental Chemistry, 21(2), 271-279.

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Quantifying Endogenous Hormones in Plant Tissues

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For quantifying endogenous hormones, five replicates of 8-weeks old root tissues from each genotype were harvested (~200 mg), snap-frozen and stored at -80 o C. Hormone extraction was performed previously described (Brenya et al., 2020; Hill et al., 2021) and analysed using the UPLC/ESI-MS/MS (Water, Milford, USA). The external standard of ABA was obtained from Sigma Aldrich. The internal standard, a deuterated compound of [ 2 H 6 ](+)-cis,trans-ABA, was obtained from Olchemim Ltd, Olomouc, Czech Republic. For the analysis of the extracts, a HALO™ C18 (Advanced Materials Technology, Inc., Wilmington, USA) column (2.1 × 75 mm, 2.7 μm) was used. Calibration curves for each analyte were generated using MassLynx 4.1™ software (Waters, USA).

Nayak J.J., Anwar S., Krishna P., Chen Z.H., Plett J.M., Foo E, & Cazzonelli C.I. (2022). Tangerine tomato roots show increased accumulation of acyclic carotenoids, less abscisic acid, drought sensitivity, and impaired endomycorrhizal colonization. Plant science : an international journal of experimental plant biology, 321.

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