Kma0021

Manufactured by Thermo Fisher Scientific

Sourced in United States

The KMA0021 is a laboratory centrifuge designed for general-purpose applications. It has a maximum speed of 6,000 rpm and a maximum RCF (Relative Centrifugal Force) of 4,150 x g. The centrifuge accommodates a variety of sample tubes and microplates. It features a digital display for speed and time control.

Automatically generated - may contain errors

Lab products found in correlation

Mcp 1 ccl2, by R&D Systems (1 mentions) Freestyle freedom light, by Abbott (1 mentions) Hk205 02, by Hycult Biotech (1 mentions)

2 protocols using kma0021

Plasma Biomarkers in Fasting Mice

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fasting (5 h) blood samples were taken from the tail vein to prepare EDTA plasma. Blood glucose was determined during blood sampling using a hand-held glucometer (Freestyle Freedom Light, Abbott Laboratories, Lake Bluff, IL, United States). Plasma insulin, leptin, adiponectin, cholesterol, triacylglycerols, ALT, CK-18M30 were determined as described (16 (link)). ELISA assays were used to measure plasma serum amyloid A (SAA; KMA0021 Thermo Fisher Scientific, Waltman, United States), MCP-1/CCL2 (DY479 R&D, Minneapolis, United States), MIF (DY1978 R&D, Fetuin A (DY1563 R&D) and LPS binding protein (LBP; HK205-02 Hycult Biotech, Uden, Netherlands) according to manufacturer’s protocol.
Lipoprotein profiles were analyzed by first separating lipoproteins into fractions using fast protein liquid chromatography (FPLC) with an AKTA apparatus (Pharmacia, Roosendaal, Netherlands), as previously described (17 (link), 18 (link)). Subsequently, total cholesterol and triacylglycerols were measured in the fractions collected for profiling with enzymatic assays (Roche diagnostics, Basel, CHF) (19 (link)–21 (link)).

Gart E., Salic K., Morrison M.C., Giera M., Attema J., de Ruiter C., Caspers M., Schuren F., Bobeldijk-Pastorova I., Heer M., Qin Y, & Kleemann R. (2022). The Human Milk Oligosaccharide 2′-Fucosyllactose Alleviates Liver Steatosis, ER Stress and Insulin Resistance by Reducing Hepatic Diacylglycerols and Improved Gut Permeability in Obese Ldlr-/-.Leiden Mice. Frontiers in Nutrition, 9, 904740.

+ Open protocol

+ Expand

Serum Amyloid A Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were euthanized 3, 7, 14, 21 and 35 days after surgery via CO₂ inhalation followed by cervical dislocation and blood was collected via cardiac puncture, from both infected mice and non-infected controls. Blood was collected retro-orbitally on day 28 after mice were anesthetized via isoflurane inhalation. Blood was centrifuged and serum stored at −80°C for future use. Serum amyloid A (SAA) levels were quantified using an ELISA kit (KMA0021; Thermo Fisher Scientific) according to the manufacturer’s instructions.

Sokhi U.K., Xia Y., Sosa B., Turajane K., Nishtala S.N., Pannellini T., Bostrom M.P., Carli A.V., Yang X, & Ivashkiv L.B. (2022). Immune Response to Persistent Staphyloccocus aureus Periprosthetic Joint Infection in a Mouse Tibial Implant Model. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(3), 577-594.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!