Iron tri acetylacetonate

Iron tri(acetylacetonate) (2 mmol), 1,2-tetradecanediol (10 mmol), oleic acid (6 mmol), oleylamine (6 mmol), dibenzyl ether, citric acid, diethyl ether, 2-methoxyethylamine, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), thioglycolic acid, hydroxylamine, and phenanthroline were all purchased from Sigma-Aldrich (St. Louis, Missouri). 1,2-dichlorobenzene and N,N’-dimethylformamide were from Acros Organics (Morris Plains, New Jersey). NH₂-PEG (550 Da, 2000 Da, 5000 Da, and 10000 Da) were all obtained from Laysan Bio, Inc. (Arab, Alabama). Slide-A-Lyzer G2 dialysis cassettes, chambered slides, bovine calf serum (BCS) were acquired from Thermo Scientific (Rockford, Illinois). Dulbecco’s Modified Eagle’s Medium (DMEM), Dulbecco’s Phosphate Buffer Saline (DPBS), penicillin, and streptomycin were purchased from Gibco Life Technologies, Inc. (NY, USA).

Iron tri(acetylacetonate), 1,2-tetradecanediol, oleic acid, oleylamine, dibenzyl ether, citric acid, diethyl ether, 2-methoxyethylamine, N-(3- Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), 1,2-dichlorobenzene, and N,N’-dimethylformamide are all purchased from Sigma-Aldrich (St. Louis, Missouri). The LIVE/DEAD Assay kit was purchased from ThermoFisher Scientific (Rockford, Illinois). Human Umbilical Cord Vascular Endothelial Cells (HUVECs) were acquired from Lonza (Portsmouth, New Hampshire). Where it was purchased, HA powder was also purchased from Sigma-Aldrich (St. Louis, Missouri). All functionalized PEG reagents are obtained from Nanocs (Boston, Massachusetts). Slide-A-Lyzer dialysis cassettes are acquired from Thermo Scientific (Rockford, Illinois). Fetal Bovine serum is obtained from Hyclone (Logan, Utah). Media used to culture cells in these experiments is also obtained from Lonza (Portsmouth, New Hampshire).