Jc 1 probe kit

Manufactured by Beyotime

Sourced in China

The JC-1 Probe Kit is a laboratory equipment used for the detection and analysis of mitochondrial membrane potential. The kit includes the JC-1 fluorescent probe, which is capable of indicating changes in the mitochondrial membrane potential of cells.

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Lab products found in correlation

Tissue mitochondria extraction kit, by Beyotime (1 mentions)

Close spelling variants of this product

JC-1 fluorescence probe kit JC-1 fluorescent probe assay kit JC-1 fluorescent probe kit JC-1 probe JC-1 kit

2 protocols using jc 1 probe kit

Ursolic Acid Impacts Breast Cancer Mitochondria

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Mitochondrial membrane potential detection assay was conducted to investigate the effect of ursolic acid on the mitochondrial membrane potential and apoptosis of breast cancer cells. Briefly, breast cancer cells were treated with ursolic acid (0~30 μM) for 48 h. Then, cells were collected and subjected to JC-1 probe staining assay using the JC-1 Probe Kit (Beyotime, C2006). JC-1 green fluorescence intensities of breast cancer cells were analyzed using the NovoCyte flow cytometer.

Wang S., Chang X., Zhang J., Li J., Wang N., Yang B., Pan B., Zheng Y., Wang X., Ou H, & Wang Z. (2021). Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling. Frontiers in Oncology, 11, 745584.

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Mitochondrial Membrane Potential Measurement

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The mitochondrial membrane potential (ΔΨm) was determined using a JC-1 probe kit (Beyotime, Shanghai, China). Firstly, a small section (50 mg) of liver was excised and weighted immediately after rat sacrifice. Liver mitochondria was obtained by a tissue mitochondria extraction kit (Beyotime, Shanghai, China). Thereafter, the purified mitochondria were stained with a 1× JC-1 working solution, and samples were detected by a fluorescence microplate. ΔΨm was calculated as the ratio of red fluorescence (JC-1 aggregates) to green fluorescence (JC-1 monomers).

Wu Y., Wang B., Tang L., Zhou Y., Wang Q., Gong L., Ni J, & Li W. (2022). Probiotic Bacillus Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model. Antioxidants, 11(2), 291.

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