Elution buffer

Manufactured by Roche

Sourced in Switzerland

Elution buffer is a laboratory reagent used to extract and purify target molecules, such as proteins or DNA, from a sample. It serves to efficiently displace and recover the desired substance from a solid support or column during the final step of the purification process. The buffer composition is designed to facilitate the release of the target analyte, allowing for its collection and further analysis or application.

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Lab products found in correlation

High pure rna isolation kit, by Roche (3 mentions) High pure filter tube, by Roche (2 mentions) Lysis buffer, by Roche (1 mentions) Magna pure lc total nucleic acid isolation kit, by Roche (1 mentions) Automated nucleic acid robotic workstation, by Roche (1 mentions) Platinum taq dna polymerase high fidelity, by Thermo Fisher Scientific (1 mentions) Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (1 mentions) Magna pure 96 instrument, by Roche (1 mentions) Magna pure 96 dna and viral na small volume kit, by Roche (1 mentions) Fta classic card, by Cytiva (1 mentions) Whatman, by Cytiva (1 mentions) Quantifiler duo dna quantification kit, by Thermo Fisher Scientific (1 mentions) Ds 11, by DeNovix (1 mentions)

Close spelling variants of this product

MagnaPure LC elution buffer (2 citations) MagNA Pure LC DNA Isolation Kit-Large Volume Elution Buffer (2 citations)

7 protocols using elution buffer

HPV DNA Extraction and Amplification

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Lysis Buffer, Wash Buffer, Elution Buffer, HPV Positive and Negative Control Kits, and all other reagents were purchased from Roche Molecular Systems Inc. Each sample had an internal control, β-globin, to monitor cell adequacy, and each run included a set of HPV Positive and Negative Controls. DNA extraction and purification were done with the Cobas x 480 Instrument according to the manufacturer's instructions. Briefly, the PreservCyt Solution samples were vortexed and placed on the sample carrier, and the reagents, e.g., Lysis Buffer, Wash Buffer, and Elution Buffer, were loaded in respective reagent reservoir carriers. After sample and reagent loading, DNA preparation was completed automatically and final DNA products were collected into a microwell plate. Subsequently, the microwell plate containing DNA was manually sealed and loaded on the Cobas z 480 Analyzer, and the amplification and hybridization were completed automatically.

Wang S., He X., Meng F., Pan Q., Zhang L, & Zeng J. (2020). Application of the Cobas 4800 System for the Detection of High-Risk Human Papillomavirus in 5650 Asymptomatic Women. BioMed Research International, 2020, 1635324.

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Quantifying SARS-CoV-2 Viral Loads

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To determine viral loads in the serum samples and tissue homogenates, we used qRT-PCR to measure viral RNA titres (serum and tissue) and TCID₅₀ titration for the calculation of infectious virus titres (serum only). Briefly, RNA was isolated from 50 μl serum or 100 μl homogenized tissue using the MagNA Pure LC Total Nucleic Acid Isolation kit (Roche) and an automated nucleic acid robotic workstation (Roche) according to the manufacturer's instructions. RNA was eluted in 100 μl elution buffer (Roche) and stored at −80 °C until assayed. RNA copy numbers were quantified using unmodified primers as described previously (Lim et al., 2013a (link)). The limit of detection of the assay was 0.95 log₁₀RNA copies.
Infectious titres in the serum were determined by log₁₀ titration of the serum samples on Vero E6 cells and calculating the TCID₅₀ using the Spearman–Kärber method (Kärber, 1931 ; Spearman, 1908 ) after the determination of CPE 5 days p.i. Initial 1 : 10 dilution of serum resulted in a limit of detection of 10^1.75 TCID₅₀ ml⁻¹.

Lim S.M., Brault A.C., van Amerongen G., Sewbalaksing V.D., Osterhaus A.D., Martina B.E, & Koraka P. (2014). Susceptibility of European jackdaws (Corvus monedula) to experimental infection with lineage 1 and 2 West Nile viruses. The Journal of general virology, 95(0 6), 1320-1329.

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Influenza A H5N1 Genome Amplification

Cited 2 times

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For the human H5N1 samples 200 µl of clinical sample was added to 400 µl lysis-binding buffer (High pure RNA isolation kit, Roche) and RNA extraction was subsequently performed using the High pure RNA isolation kit (Roche) with an on-column DNase treatment according to the manufacturer's protocol. Total RNA was eluted in a volume of 50 µl elution buffer (Roche) and directly used for reverse transcription and amplification. cDNA was synthesized using the Uni12M primer (AGCRAAAGCAGG) [31 (link)] and the Superscript III First-Strand Synthesis System according to the manufacturer's protocol (Invitrogen), followed by amplification of the whole genome in an overlapping amplicon approach using degenerative primer sets (primer sequences available upon request) [18 (link),32 (link)]. PCR reactions were performed using Platinum Taq DNA Polymerase High Fidelity (Invitrogen). Thermal cycling conditions were: denaturation at 95°C for 5 min; 40 cycles of 95°C for 30 s, 50°C for 30 s, 68°C for 1 min; and final extension at 68°C for 5 min.

Welkers M.R., Pawestri H.A., Fonville J.M., Sampurno O.D., Pater M., Holwerda M., Han A.X., Russell C.A., Jeeninga R.E., Setiawaty V., de Jong M.D, & Eggink D. (2019). Genetic diversity and host adaptation of avian H5N1 influenza viruses during human infection. Emerging Microbes & Infections, 8(1), 262-271.

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Automated DNA Extraction from Oral Rinse

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Oral rinse samples were thawed at room temperature, vortexed and 500 μL were used for automated DNA extraction. Extraction was done using the MagNa Pure 96 DNA and Viral NA Large Volume Kit on the platform of the MagNa Pure 96 instrument in combination with the ‘Pathogen universal 500’ protocol. DNA was eluted in 50 μL elution buffer (Roche, Mannheim, Germany) and stored at -20°C until further processing.

Lupato V., Holzinger D., Höfler D., Menegaldo A., Giorgi Rossi P., Del Mistro A., Da Mosto M.C., Pawlita M, & Boscolo-Rizzo P. (2017). Prevalence and Determinants of Oral Human Papillomavirus Infection in 500 Young Adults from Italy. PLoS ONE, 12(1), e0170091.

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Nucleic Acid Extraction from Primary Samples

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When applied, the nucleic acids were extracted from 200 μL of the primary sample using a MagNA Pure 96 Instrument (Roche Diagnostics, Basel, Switzerland) and MagNA Pure 96 DNA and Viral NA Small Volume Kit (protocol Viral NA Universal version 4.0, Roche Diagnostics, Basel, Switzerland), with elution in 100 μL of elution buffer (Roche Diagnostics, Basel, Switzerland).

Barra G.B., Santa Rita T.H., Mesquita P.G., Jácomo R.H, & Nery L.F. (2021). Overcoming Supply Shortage for SARS-CoV-2 Detection by RT-qPCR. Genes, 12(1), 90.

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RNA Extraction from Bloodstains on FTA Cards

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Bloodstains collected from 10 patients on pieces of FTA Classic Cards (Whatman), approximately in 0.5 cm 2 in size, were incubated in the Lysis/Binding w kolumnie filtracyjnej High Pure Filter Tube (Roche Diagnostics), traktowano DNAzą oraz uzyskiwano RNA zgodnie z protokołem podanym przez producenta zestawu High Pure RNA Isolation Kit (Roche Diagnostics). RNA zawieszano w 50 µl Elution Buffer (Roche Diagnostics).

Skonieczna K., Styczyński J., Krenska A., Wysocki M., Jakubowska A, & Grzybowski T. (2016). RNA isolation from bloodstains collected on FTA cards - application in clinical and forensic genetics. Archiwum medycyny sadowej i kryminologii, 66(4).

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RNA Extraction and Purification Protocol

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Oczyszczone po traktowaniu DNAzą ekstrakty RNA oceniano spektrofotometrycznie z wykorzystaniem aparatu DS-11 (DeNovix).
Obecność zanieczyszczeń RNA w postaci DNA oceniano z wykorzystaniem zestawu Quantifiler® Duo DNA Quantification Kit (Thermo Fisher Scien-Buffer (Roche Diagnostics) at room temperature for 30 minutes. In the next step, they were placed in a filtration column (High Pure Filter Tube (Roche Diagnostics)) and treated with DNAse, producing RNA according to the protocol specified by the manufacturer of the High Pure RNA Isolation Kit (Roche Diagnostics). RNA was suspended in 50 µl of the Elution Buffer (Roche Diagnostics).

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