Pdhk2

Manufactured by Novus Biologicals

PDHK2 is a protein expressed by the PDHK2 gene. It is a member of the pyruvate dehydrogenase kinase family, which is responsible for regulating the activity of the pyruvate dehydrogenase complex.

Automatically generated - may contain errors

Lab products found in correlation

Pdhk4, by Novus Biologicals (3 mentions) Pdhk3, by Novus Biologicals (3 mentions) Hif 1α, by BD (3 mentions) Gapdh, by Abcam (2 mentions) Pdha1, by Abcam (2 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions) Hif 2α, by Novus Biologicals (1 mentions) Complete mini protease inhibitor cocktail, by Roche (1 mentions) Chemidoc, by Bio-Rad (1 mentions)

3 protocols using pdhk2

PDH Regulatory Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed with RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β -glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride (PMSF), and complete mini protease inhibitor cocktail (Roche)), 20–50 μg of total proteins electrophoresed and blotted to PVDF, and probed with anti: PDHA1 (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), and GAPDH (abcam). Primary antibodies were detected with fluorochrome-labeled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.

Echeverri Ruiz N.P., Mohan V., Wu J., Scott S., Kreamer M., Benej M., Golias T., Papandreou I, & Denko N.C. (2021). Dynamic regulation of mitochondrial pyruvate metabolism is necessary for orthotopic pancreatic tumor growth. Cancer & Metabolism, 9, 39.

+ Open protocol

+ Expand

Protein Phosphorylation and Enzyme Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysates were generated in RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β-glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride, and Complete Mini protease inhibitor cocktail (Roche)), 20–50 μg of total proteins electrophoresed and blotted to PVDF. Antibodies used were for total PDH E1α (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), HIF2α (1:1000, Novus), tubulin (1:1000, Santa Cruz). Primary antibodies were detected with HRP secondaries (1:4000, Santa Cruz) and visualized with ECL (Amersham) on film or ChemiDoc (Bio-Rad), or with fluorochrome labelled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.

Golias T., Papandreou I., Sun R., Kumar B., Brown N.V., Swanson B.J., Pai R., Jaitin D., Le Q.T., Teknos T.N, & Denko N.C. (2016). Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Scientific Reports, 6, 31146.

+ Open protocol

+ Expand

Characterizing PDH Complex Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed with RIPA buffer containing protease and phosphatase inhibitors (5 mM sodium fluoride, 2 mM β -glycerophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride (PMSF), and complete mini protease inhibitor cocktail (Roche)), 20-50 μ g of total proteins electrophoresed and blotted to PVDF, and probed with anti: PDHA1 (1:6000, MitoSciences), pSer293-E1α (1:1000, EMD Chemicals), pSer300-E1α (1:1000, EMD Chemicals), pSer232-E1α (1:3000, EMD Chemicals), PDHK1 (1:4000, Assay Designs), PDHK2 (1:500, Novus), PDHK3 (1:1000, Novus), PDHK4 (1:1000, Novus), PDP1 (1:1000, Sigma Aldrich), HIF1α (1:1500, BD), GAPDH (abcam). Primary antibodies were detected with fluorochrome labelled secondary antibodies (Li-Cor) visualized on a Li-Cor Odyssey.

Ruiz N.P.E., Mohan V., Wu J., Scott S., Kreamer M., Benej M., Papandreou I., & Denko N. (2021). Dynamic regulation of mitochondrial pyruvate metabolism Is necessary for orthotopic pancreatic tumor growth.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!