Pfocts

Manufactured by Merck Group

Sourced in Germany

PFOCTS is a specialized laboratory equipment produced by Merck Group. It serves as a tool for performing various scientific experiments and analyses. The core function of PFOCTS is to facilitate precise and controlled measurements, data collection, and sample processing in a laboratory setting. No further details or interpretation on the intended use of this product are provided.

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Lab products found in correlation

5 protocols using pfocts

Minimally Invasive Sub-Retinal Cell Delivery

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We fabricated a glass needle delivery system for minimally invasive sub-retinal
implantation of cells. Glass capillaries (OD: 1.2 mm, ID: 0.68 mm, WPI) were
heated to 80°C with a puller, stretched, and ground with a grinder (Micro
Grinder EG-400, Narishige, Tokyo, Japan) to a tip angle of 30°. The glass needle
was then coated with either trichloro(1H,1H,2H,2H,2H-perfluorooctyl) silane
(PFOCTS, Sigma Aldrich, Munich, Germany) or MPC (Tokyo Chemical Industry Co.,
Ltd, Tokyo, Japan). Thus, PFOCTS was placed in a sealed container with the glass
needle and allowed to vapor-deposit for 24 h, whereas MPC was adjusted to 0.5
wt.% in ethanol and coated using aspiration and ejection with the glass needle.
DPBS(-) and hyaluronic acid sodium salt (NaHA, Combi-Blocks, Inc., San Diego,
CA, USA) adjusted to 0.5, 1.0, and 1.5 w/v% in DPBS(-) were used as a delivery
solution for cell transplantation. A silicon tube was used to connect the glass
needle to a syringe to create a delivery system. The syringe and silicon tube
were filled with air or medical olive oil, and the cells on nanosheet were
delivered at the tip of the needle.

Yamashita K., Ostrovidov S., Raut B., Hori T., Nashimoto Y., Nagai N., Abe T, & Kaji H. (2023). Minimally Invasive Sub-Retinal Transplantation of RPE-J Cells on a Biodegradable Composite PCL/Collagen Nanosheet. Cell Transplantation, 32, 09636897231165117.

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Microfluidic Vesicle Formation and Characterization

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All materials were used as purchased unless noted otherwise. 1‐heptanol (99%), 1‐octanol (99%), 1‐nonanol (98%), 1‐decanol (98%), 1‐undecanol (99%), Poly(diallyldimethylammonium chloride) (PDADMAC), poly(sodium 4‐styrenesulfonate) (PSS), hydrogen peroxide (H₂O₂) (30 wt%), hydrochloric acid (HCl) (37 wt%), bovine serum albumin (BSA), cholesterol, Ficoll PM70, 3‐(trimethoxysilyl)propyl acrylate (TMSPMA) (92% with 100 ppm BHT), trichloro(1H,1H,2H,2H‐perfluorooctyl)silane (PFOCTS) (97%), Pluronic F‐127 and sodium chloride (NaCl) were purchased from Sigma Aldrich. 1‐Palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC), 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phospho‐(1’‐rac‐glycerol) (sodium salt) (POPG) was obtained from Avanti polar lipids. DiIC₁₈(5) solid (1,1’‐Dioctadecyl‐3,3,3’,3’‐Tetramethylindodicarbocyanine, 4‐Chlorobenzenesulfonate Salt) (DiD) was purchased from ThermoFischer Scientific. Poly(dimethylsiloxane) (PDMS) and curing agent were obtained as SYLGARD 184 Silicone Elastomer Kit from DOW Corning.

Guerzoni L.P., de Goes A.V., Kalacheva M., Haduła J., Mork M., De Laporte L, & Boersma A.J. (2022). High Macromolecular Crowding in Liposomes from Microfluidics. Advanced Science, 9(27), 2201169.

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Microfluidic Chip Fabrication with PDMS

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Channels
containing microfluidic chips were fabricated by standard soft lithography
process using PDMS (poly(dimethylsiloxane), Sylgard 184, Dow Corning).
Briefly, all molds were silanized under vacuum using trichloro (1H,1H,2H,2H-perfluorooctyl)silane
(PFOCTS) (Sigma-Aldrich U.K.). PDMS was mixed with a curing agent
at a ratio of 10:1, degassed for 30 min and then poured onto the masters,
a silicon wafer, an SLA 3D-printed mold, or a copper clad laminate
wafer. The wafers were then placed on a hot plate at 80° for
2 h to cure. PDMS replicas were peeled from the masters, cut to size,
and placed on a microscope slide for inspection under a microscope.
3 μm polystyrene fluorescent (FLASH-red) microspheres were purchased
from Bangs Laboratories.

Todd D, & Krasnogor N. (2023). Homebrew Photolithography for the Rapid and Low-Cost, “Do It Yourself” Prototyping of Microfluidic Devices. ACS Omega, 8(38), 35393-35409.

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Fabrication of Through-Holes and Post Arrays

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Polydimethylsiloxane (PDMS; Sylgard 184, Dow Corning), OSTEMER (OSTEMER 322 Crystal Clear, Mercene Labs), and poly(vinyl alcohol) (PVA; M_w 89 000–98 000, 99+% hydrolyzed, 341584, Sigma Aldrich) were used to fabricate through-holes and post arrays. Chlorotrimethylsilane (TMSCI; 386529, Sigma-Aldrich), and trichloro(1H,1H,2H,2H-perfluorooctyl)silane (PFOCTS; 448931, Sigma-Aldrich) were used for the surface modification of the through-holes and post arrays. AgNW dispersions (20 nm × 20 μm, Novarials) and PEDOT:PSS (Sigma Aldrich) were used as provided by the manufacturer. Polycarbonate (PC), PET, and glass were used as substrates.

Chae Y., Bae J., Lim K, & Kim T. (2022). Performance characterization of transparent and conductive grids one-step-printed on curved substrates using template-guided foaming. RSC Advances, 12(43), 27846-27854.

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PNIPAM Hydrogel Synthesis and Characterization

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N,N’-methylenebis(acrylamide) (BIS; 99%, Sigma Aldrich), ammonium persulfate (APS, Sigma Aldrich, 98%), potassium persulfate (KPS, Merck, >99%), N,N’-(1,2-dihydroxyethylene)bisacrylamide (DHEA, Merck, 97%), methacrylic acid (Merck, 99%), sodium periodate (99.8%), Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (PFOCTS, 97%, Sigma Aldrich), ethanol (Sigma Aldrich, >99.5%), linear poly(N-Isopropylacrylamide) (P NIPAM, 10 kD, Sigma Aldrich) and Nile Red (>98%, Sigma Aldrich), hexane (≥99%, Sigma Aldrich) were used as received. N-Isopropylacrylamide (NIPAM; 97%, Sigma Aldrich) was purified by recrystallization from hexane (95%, Sigma Aldrich). Dodecane (99%, Acros organics) was passed through an alumina column twice. Water was double deionized using a Milli-Q system (18.2 MΩ·cm).

Rey M., Kolker J., Richards J.A., Malhotra I., Glen T.S., Li N.Y., Laidlaw F.H., Renggli D., Vermant J., Schofield A.B., Fujii S., Löwen H, & Clegg P.S. (2023). Interactions between interfaces dictate stimuli-responsive emulsion behaviour. Nature Communications, 14, 6723.

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