Tibial samples from 4-month-old female mice were dissected free of soft tissue, fixed overnight in 70% ethanol and analyzed by micro-CT with a Quantum GX2 scanner (PerkinElmer, USA). For tibia reconstruction, the trabecular bone volume of interest (VOI) was drawn to include 200 slices of metaphyseal spongiosa starting from 0.9 mm below the growth plate. The trabecular bone mineral density (Tb.BMD), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp) and trabecular bone volume/tissue volume (BV/TV) as well as the cortical bone mineral density (Ct.BMD) and cortical bone thickness (Ct.Th) were calculated using the software supplied with the instrument.
Quantum gx2 scanner
Manufactured by PerkinElmer
Sourced in United States
The Quantum GX2 scanner is a medical imaging device designed for in vivo small animal imaging. It utilizes x-ray technology to produce high-resolution images of small animals for research purposes.
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Lab products found in correlation
4 protocols using quantum gx2 scanner
1
Murine Bone Microarchitectural Analysis
2
Micro-CT Analysis of Vertebral Degeneration
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The scanning protocol included an isometric resolution of 15 μm, with X-ray energy settings of 70 kV and 200 μA. The microstructure of the vertebrae was measured through a Quantum GX2 scanner (PerkinElmer, USA). Prior to histological processing, samples were fixed in paraformaldehyde and used for micro-CT. The scanned images from each group were evaluated at the same threshold to allow 3-dimensional structural reconstruction of each sample. The degenerative score followed the methods described in a previous study [62 (link)].
3
Micro-CT Analysis of Vertebral Degeneration
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The scanning protocol included an isometric resolution of 15 μm, with X-ray energy settings of 70 kV and 200 μA. The microstructure of the vertebrae was measured through a Quantum GX2 scanner (PerkinElmer, USA). Prior to histological processing, samples were fixed in paraformaldehyde and used for micro-CT. The scanned images from each group were evaluated at the same threshold to allow 3-dimensional structural reconstruction of each sample. The degenerative score followed the methods described in a previous study [62 (link)].
4
Evaluating Vertebral Microstructure via Micro-CT
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The scanning protocol included an isometric resolution of 15 μm, as well as X-ray energy settings of 70 kV and 200 A. A Quantum GX2 scanner (PerkinElmer, USA) was used to measure the microstructure of the vertebrae. Before histological processing, samples were fixed in paraformaldehyde and micro-CT was performed. Each group’s scanned pictures were analyzed at the same threshold to enable for 3-dimensional structural reconstruction of each sample. The degenerative score was calculated using the procedures outlined in a previous study.68 (link)
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