Dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium bromide (HNSB), deuterium oxide, pepsin, imidazole, 3-morpholinopropanesulfonic acid (MOPS), potassium acetate, potassium bromide, urea, zinc sulfate, deuterium oxide, tris(2-carboxyethyl)phosphine (TCEP), and dithiothreitol (DTT) were obtained from Sigma-Aldrich (St. Louis, MO). urea was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Trypsin was purchased from Promega (Madison, WI). Tris(hydroxymethyl)-aminomethane (Tris) and tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) were purchased from EM Science (Gladstone, NJ). Human β2m that was purified from human urine was purchased from Lee Biosolutions (St. Louis, MO). Ammonium acetate, methanol, acetonitrile, glacial acetic acid, copper sulfate, and nickel sulfate were obtained from Fisher Scientific (Fair Lawn, NJ). Centricon molecular weight cutoff (MWCO) filters were obtained from Millipore (Burlington, MA). Deionized water was prepared from a Millipore (Burlington, MA) Simplicity 185 water purification system.
Nickel sulfate
Manufactured by Thermo Fisher Scientific
Nickel sulfate is a chemical compound with the formula NiSO4. It is a green crystalline solid that is soluble in water. Nickel sulfate is commonly used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Potassium bromide, by Merck Group (2 mentions)
Zinc sulfate, by Thermo Fisher Scientific (2 mentions)
Ampicillin, by Thermo Fisher Scientific (2 mentions)
Potassium acetate, by Merck Group (1 mentions)
Copper sulfate, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (1 mentions)
3 morpholinopropanesulfonic acid mops, by Merck Group (1 mentions)
Centricon molecular weight cutoff mwco filters, by Merck Group (1 mentions)
Simplicity 185 water purification system, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Deuterium oxide, by Merck Group (1 mentions)
Zinc sulfate, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine tcep, by Merck Group (1 mentions)
Trypsin, by Promega (1 mentions)
5 protocols using nickel sulfate
1
Protein Purification and Analysis Protocol
2
Synthesis of Chitosan Oligosaccharide-Based Nanomaterials
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Chitosan oligosaccharide
lactate, sodium borohydride, and silver nitrate were purchased from
Aldrich and were used as received. Potassium iodide, sodium chloride,
sodium fluoride, potassium bromide, potassium chloride, aluminum oxide,
sodium nitrate, potassium cyanide, sodium nitrite, hydrochloric acid,
sodium hydroxide, sodium sulfate, sodium thiosulfate, sodium acetate,
sodium carbonate, potassium thiocyanate, monosodium phosphate, and
disodium phosphate were purchased from Aldrich and used as received
without further purification. Magnesium acetate, iron(III) chloride,
iron(II) chloride, zinc sulfate, nickel sulfate, magnesium sulfate,
and copper nitrate were purchased from Fisher and used as received
without further purification. Dialysis tubes with a 29.3 mm diameter
and 3.5 kDa molecular weight cutoff (MWCO) were obtained from Fisher
Scientific. A phosphate buffer solution (pH 3.0 to pH 13) was prepared
with monosodium phosphate and disodium phosphate (0.2 M), further
adjusted with sodium hydroxide and hydrochloric acid. All glassware
was thoroughly washed with (3:1 ratio of HCl/HNO3) aqua
regia and was then rinsed with ultrapure water prior to use. Ultrapure
water was used in all of the experiments.
lactate, sodium borohydride, and silver nitrate were purchased from
Aldrich and were used as received. Potassium iodide, sodium chloride,
sodium fluoride, potassium bromide, potassium chloride, aluminum oxide,
sodium nitrate, potassium cyanide, sodium nitrite, hydrochloric acid,
sodium hydroxide, sodium sulfate, sodium thiosulfate, sodium acetate,
sodium carbonate, potassium thiocyanate, monosodium phosphate, and
disodium phosphate were purchased from Aldrich and used as received
without further purification. Magnesium acetate, iron(III) chloride,
iron(II) chloride, zinc sulfate, nickel sulfate, magnesium sulfate,
and copper nitrate were purchased from Fisher and used as received
without further purification. Dialysis tubes with a 29.3 mm diameter
and 3.5 kDa molecular weight cutoff (MWCO) were obtained from Fisher
Scientific. A phosphate buffer solution (pH 3.0 to pH 13) was prepared
with monosodium phosphate and disodium phosphate (0.2 M), further
adjusted with sodium hydroxide and hydrochloric acid. All glassware
was thoroughly washed with (3:1 ratio of HCl/HNO3) aqua
regia and was then rinsed with ultrapure water prior to use. Ultrapure
water was used in all of the experiments.
3
His-tagged Protein Purification Protocol
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IPTG and dithiothreitol (DTT) were purchased from Gold Biotechnology. Ampicillin, nickel sulfate, potassium phosphate (KH2PO4 and K2HPO4), citric acid, sodium citrate (dihydrate), tris base, imidazole, tryptone, yeast extract and ultra-pure urea were purchased from Fisher. His-bind resin was purchased from VWR.
4
Nickel Sulfate and SB Effects on Rat Reproductive Outcomes
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The rats were divided into four groups for thirty-day administration. As the control group the rats were injected with 1 ml isotonic saline solution, intraperitoneally (Group1, n=6). Nickel sulfate (Acros Organics) was given alone at doses of 5 mg/kg/day (Su et al. 2011) to Group 2 (n=6), respectively. In the SB (Sigma Chemical) group (Group 3, n=6) the rats received only daily SB in 150 mg/kg/day dose (Oufi and AlShawi 2014) . SB, 150 mg/kg/day was added to Nickel sulfate 5 mg/kg/ /day in Group 4 (n=6). Nickel sulfate was always diluted with 1 ml isotonic saline solution and administered intraperitoneally and SB was suspended in a 0.3% carboxymethylcellulose (CMC) (Sigma Chemical) solution and administered orally (Lu et al. 2009 ). The rats were sacrificed by cervical dislocation on the thirty-first day. Following collection of testes and sperm samples, the serum was prepared by centrifugation (1500xg, 15 min, 4°C), frozen and stored at 20°C; the sperm samples obtained from fresh cauda epididymis were held in 0.9% NaCl and were processed according to the method described previously (Muralidhara and Narasimhamurthy 1991) to determine the sperm counts. Motility, membrane, and acrosome integrity of the sperms were checked using the method described previously (Sen et al. 2017) .
5
Characterization of S. aureus Newman and MRSA Strains
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S. aureus strain Newman was verified by whole genome sequencing. The MRSA strains used in this study were obtained in a deidentified manner from UAB Laboratory Medicine after confirmation of their drug resistance. The transposon mutants and the parent JE2 were obtained from BEI as part of the Nebraska Transposon Mutant Library. Bacteria were inoculated from stocks stored at −80 °C into Mueller Hinton media (Oxoid LTD) and incubated overnight at 37 °C with shaking at 180 rpm. THP-1 monocytes and PBMCs were cultured in Roswell Park Memorial Institute (RPMI) media (Corning) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, Inc.), glutamine, penicillin and streptomycin, and incubated at 37 °C in the presence of 5% CO2. APT-6K was purchased from ChemBridge Corp., reconstituted and aliquoted in DMSO at a 10 mM stock concentration, and stored at −80 °C. Ampicillin (Fisher Scientific) was reconstituted and aliquoted in ddH2O at 10 mg/mL and stored at −80 °C. Copper sulfate (Acros Organics), zinc sulfate (Fisher Scientific), manganese sulfate (Alfa Aesar), cobalt sulfate (Alfa Aesar), nickel sulfate (Acros Organics), and iron chloride (Acros Organics) were reconstituted and aliquoted in ddH2O at 100 mM and stored at 4 °C.
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