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KAT2B is a protein that functions as a histone acetyltransferase, which catalyzes the acetylation of histones. Histones are proteins that help package DNA in the cell nucleus, and acetylation of histones can regulate gene expression.

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Lab products found in correlation

Histone h3, by Cell Signaling Technology (3 mentions) Kat6a, by Abbkine (2 mentions) Kat2a, by Cell Signaling Technology (2 mentions) Tif1β, by Cell Signaling Technology (2 mentions) Flag m2 antibody, by Cell Signaling Technology (2 mentions) Tif1α, by Abcam (2 mentions) Ncoa1, by Cell Signaling Technology (2 mentions) Ncoa2, by Cell Signaling Technology (2 mentions) Tif1γ, by Cell Signaling Technology (2 mentions) Ncoa3, by Cell Signaling Technology (2 mentions) H3k9ac, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Phosphate inhibitor cocktail, by Merck Group (1 mentions) Glut4, by Cell Signaling Technology (1 mentions) Glut1, by Cell Signaling Technology (1 mentions) Glut3, by Abcam (1 mentions) Ac h3k27, by Cell Signaling Technology (1 mentions) Histone h4, by Cell Signaling Technology (1 mentions) Ac h3k9, by Cell Signaling Technology (1 mentions) Ach3k14, by Cell Signaling Technology (1 mentions)

7 protocols using kat2b

1

Neutrophil Protein Isolation and Western Blot

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Neutrophils (5 × 107 cells/ml) were suspended in 500 μl of 1× radioimmunoprecipitation assay lysis buffer (Bio Basics) supplemented with 0.2% Triton X-100 (Sigma-Aldrich), a protease inhibitor cocktail (Sigma-Aldrich), and a phosphate inhibitor cocktail (Sigma-Aldrich), 20 μM phenylmethylsulfonyl fluoride (Sigma-Aldrich), leupeptin (10 mg/ml; Sigma-Aldrich), and aprotinin (Sigma-Aldrich) for 30 min. The proteins were extracted via centrifugation at 18,000g for 30 min at 4 °C. Total proteins (60 μg) were resolved using SDS-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes, blocked with 5% skim milk, and probed with primary antibodies against GLUT1 (Cell Signaling Technology), GLUT3 (Abcam), GLUT4 (Cell Signaling Technology), ACLY (Lifespan Biosciences), PDH (Cell Signaling Technology), histone H3 (Cell Signaling Technology), histone H4 (Cell Signaling Technology), AcH3K9 (Cell Signaling Technology), AcH3K14 (Cell Signaling Technology), AcH3K27 (Cell Signaling Technology), AcH4K8 (Cell Signaling Technology), P300/CBP-associated factor (PCAF) (KAT2B; Cell Signaling Technology), retinoblastoma binding protein (RBAP) (HAT1; Cell Signaling Technology), and β-actin (Santa Cruz Biotechnology).
Shrestha S., Lee Y.B., Lee H., Choi Y.K., Park B.Y., Kim M.J., Youn Y.J., Kim S.H., Jung S.J., Song D.K., Jin H.K., Bae J.S., Lee I.K., Jeon J.H, & Hong C.W. (2024). Diabetes Primes Neutrophils for Neutrophil Extracellular Trap Formation through Trained Immunity. Research, 7, 0365.
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2

RIP and RNA Pull-Down Assay Protocols

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RIP and RNA pull-down assays were performed as we previously described [44 (link)]. Antibodies used for RIP are displayed as follows: WDR5 (#13105,1:50, Cell Signaling Technology), KAT2B (#3378, 1:50, Cell Signaling Technology), KAT5 (#12058,1:50, Cell Signaling Technology), KAT8 (#46862, 1:50, Cell Signaling Technology), KAT6A (ABP59003, 1:100, Abbkine), and KAT2A (#3305S, 1:50, Cell Signaling Technology).
Wang Y., Chen W., Lian J., Zhang H., Yu B., Zhang M., Wei F., Wu J., Jiang J., Jia Y., Mo F., zhang S., Liang X., Mou X, & Tang J. (2019). The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α. Cell Death and Differentiation, 27(2), 695-710.
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3

Immunoprecipitation of β-catenin, KAT2B, and CBP

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Nuclear extracts were prepared for IP using a Nuclear Extraction Kit (Thermo Scientific). IP assays were performed as described previously41 (link). Mouse monoclonal antibodies against β-catenin (Abcam), KAT2B (Santa Cruz) and CBP (Abcam) were used for IP assays. Immunoblots were performed using rabbit polyclonal antibodies against active β-catenin, KAT2B, and CBP (Cell Signaling Technology), as described previously42 (link).
Suzuki A., Minamide R, & Iwata J. (2018). The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Scientific Reports, 8, 15057.
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4

Immunofluorescence Analysis of Myogenesis

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Immunofluorescence analysis was performed, as described previously3 (link), using the following antibodies: mouse monoclonal antibody against active β-catenin (Millipore), rabbit polyclonal antibodies against KAT2B (Cell Signaling Technology) and CBP (Cell Signaling Technology) and mouse monoclonal antibodies against myosin heavy chain (MYH) (Sigam-Aldrich) and MyoD1 (Thermofisher). Confocal images were obtained with a confocal microscope (C2, Nikon). Fluorescent images of MYH immunostaining were captured by an inverted fluorescence microscope (IX73, Olympus). A total of 10 fields, randomly selected from three independent experiments, were used for the quantification of the length of myotubes and fusion index.
Suzuki A., Minamide R, & Iwata J. (2018). The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Scientific Reports, 8, 15057.
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5

Comprehensive Immunoprecipitation and Western Blotting Protocol

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Immunoprecipitation (IP) and Western blotting (WB) assays were conducted as previously described (19 (link)). The specific antibodies used were as follows: KAT2B (#3378, 1:1000, Cell Signaling Technology), H3K9ac (#9649, 1:1000, Cell Signaling Technology), H3K14ac (#26828, 1:1000, Cell Signaling Technology), H3K9ac (#9649, 1:1000, Cell Signaling Technology), Histone H3 (#4499, 1:1000, Cell Signaling Technology), TIF1α (ab38264, 1:1000, Abcam), TIF1β (#4124, 1:1000, Cell Signaling Technology), TIF1γ (#13387, 1:1000, Cell Signaling Technology), NCOA1 (#20301, 1:1000, Cell Signaling Technology), NCOA2 (#96687, 1:1000, Cell Signaling Technology), NCOA3 (#2126, 1:1000, Cell Signaling Technology), RBM3 (ab211356, 1:1000, Abcam), YAP (#14074, 1:1000, Cell Signaling Technology), Flag M2 antibody (#14793, 1:1000, Cell Signaling Technology), HA antibody (#2367, 1:1000, Cell Signaling Technology), and β-actin (#4970, 1:1000, Cell Signaling Technology).
Fang M., Zhang M., Wang Y., Wei F., Wu J., Mou X., Zhang Y., Liang X, & Tang J. (2020). Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Frontiers in Oncology, 10, 601055.
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6

Comprehensive Western Blotting Antibodies

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Western blot was conducted as we previously described [43 (link)]. The antibodies for western blotting are listed as follows: β-actin (#4970, 1:1000, Cell Signaling Technology), KAT2A (#3305S, 1:1000, Cell Signaling Technology), HIF-1α (#36169, 1:1000, Cell Signaling Technology), H3K9ac (#9649, 1:1000, Cell Signaling Technology), Histone H3 (#4499, 1:1000, Cell Signaling Technology), TIF1γ (#13387, 1:1000, Cell Signaling Technology), TIF1β (#4124, 1:1000, Cell Signaling Technology), TIF1α (ab38264, 1:1000, Abcam), NCOA1 (#20301, 1:1000, Cell Signaling Technology), NCOA2 (#96687, 1:1000, Cell Signaling Technology), NCOA3 (#2126, 1:1000, Cell Signaling Technology), NF90 (sc-377406, 1:500, Santa Cruz Biotechnology), WDR5 (#13105,1:1000, Cell Signaling Technology), KAT2B (#3378, 1:1000, Cell Signaling Technology), KAT5 (#12058,1:1000, Cell Signaling Technology), KAT8 (#46862, 1:1000, Cell Signaling Technology), and KAT6A (ABP59003, 1:1000, Abbkine).
Wang Y., Chen W., Lian J., Zhang H., Yu B., Zhang M., Wei F., Wu J., Jiang J., Jia Y., Mo F., zhang S., Liang X., Mou X, & Tang J. (2019). The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α. Cell Death and Differentiation, 27(2), 695-710.
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7

RNA-Protein Interaction Analysis

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RNA pull-down and RNA immunoprecipitation (RIP) assays were performed as previously shown (20 (link)). The specific antibodies used were as follows: KAT2B (#3378, 1:1000, Cell Signaling Technology) and Flag M2 antibody (#14793, 1:1000, Cell Signaling Technology).
Fang M., Zhang M., Wang Y., Wei F., Wu J., Mou X., Zhang Y., Liang X, & Tang J. (2020). Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Frontiers in Oncology, 10, 601055.
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