Pierce dna coating solution

Manufactured by Thermo Fisher Scientific

The Pierce DNA Coating Solution is a laboratory reagent designed to coat surfaces with DNA. It provides a stable and uniform DNA coating on various materials, enabling surface-based DNA applications. The solution is optimized for binding and retaining DNA on the coated surface.

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Lab products found in correlation

Maxisorp 96 well plate, by Thermo Fisher Scientific (1 mentions) Calf thymus dsdna, by Merck Group (1 mentions)

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DNA coating solution (3 citations)

2 protocols using pierce dna coating solution

DNA Coating for Immunoassay Development

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Maxisorp 96 well plates (Thermo Scientific) were coated overnight at 4 °C with calf thymus dsDNA (Sigma-Aldrich) diluted in TE buffer (Tris-HCl 10 mM, EDTA 1 mM pH 9) at a concentration of 2 µg/mL and diluted in the same volume of Pierce DNA coating solution (Thermo Scientific) to obtain a final concentration of 1 µg/mL of dsDNA. The same protocol as for anti-RNP IgG was then followed but the PBS-T contained 0.05% of Tween-20.

TCHEN J., SIMON Q., CHAPART L., THAMINY M.K., VIBHUSHAN S., SAVEANU L., LAMRI Y., SAIDOUNE F., PACREAU E., PELLEFIGUES C., BEX-COUDRAT J., KARASUYAMA H., MIYAKE K., HIDALGO J., FALLON P.G., PAPO T., BLANK U., BENHAMOU M., HANOUNA G., SACRE K., DAUGAS E, & CHARLES N. (2024). PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus. Nature Communications, 15, 3389.

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Quantitative Measurement of DNA Damage

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Enzyme immunoassay was performed using the anti-CPD (MC-062) and anti-6–4 PP (KTM-50) antibodies. Briefly, the heat-denatured genomic DNA was coated onto the microplate wells with Pierce DNA Coating Solution (17250, Thermo Fisher Scientific Inc.). After washing and blocking by PBS with the purified anti-mouse IgG mAb of 2 µg mL⁻¹, the specific antibodies of anti-CPD (MC-062) and anti-6–4 PPs (KTM-50) were diluted 1:1000 in blocking buffer and added to each well. Optical density at 405 nm was determined.

Zhu B., Chen S., Wang H., Yin C., Han C., Peng C., Liu Z., Wan L., Zhang X., Zhang J., Lian C.G., Ma P., Xu Z.X., Prince S., Wang T., Gao X., Shi Y., Liu D., Liu M., Wei W., Wei Z., Pan J., Wang Y., Xuan Z., Hess J., Hayward N.K., Goding C.R., Chen X., Zhou J, & Cui R. (2018). The protective role of DOT1L in UV-induced melanomagenesis. Nature Communications, 9, 259.

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