Total RNA was isolated from purified T cells with RNeasy Plus Micro Kit (QIAGEN). The isolated RNA was transcribed into cDNA using the using the RNA to cDNA premix (Clontech) according to the manufacturer’s instructions. SYBR green was purchased from Roche, and the assays were performed on 96-well reaction plates (Invitrogen). The real-time PCR was performed on StepOnePlus system (Invitrogen). In all experiments, β-actin was used as reference gene to normalize gene expression. Primers are shown in Supplemental Table 2 .
Rna to cdna premix
Manufactured by Takara Bio
The RNA to cDNA premix is a reagent kit designed for the reverse transcription of RNA to complementary DNA (cDNA). It contains all the necessary components, such as reverse transcriptase enzyme, buffer, and primers, to efficiently convert RNA into cDNA in a single-step reaction.
Automatically generated - may contain errors
2 protocols using rna to cdna premix
1
RNA Isolation and qPCR Analysis of T Cells
2
Quantitative RT-PCR Analysis of T-Cell Markers
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