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Elisa quantification kit

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The ELISA quantification kit is a laboratory tool used to detect and measure the concentration of specific proteins or other analytes in a sample. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to quantify the target analyte. The kit provides the necessary reagents and protocol to perform the ELISA analysis.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Aanalyst 400, by PerkinElmer (1 mentions) Gen5 version 2, by Agilent Technologies (1 mentions) 903 protein saver cards, by GE Healthcare (1 mentions) Keratinocyte sfm, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Tmb chromogen, by Thermo Fisher Scientific (1 mentions) Nunc immuno plate, by Thermo Fisher Scientific (1 mentions)

11 protocols using elisa quantification kit

1

Quantifying Protein Levels in BALF

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Total protein concentrations in the cell-free BAL supernatant were determined using the Pierce BCA Protein Assay Kit (WJ334006, Thermo Fisher Scientific, Berkeley, MO). Albumin levels were determined with an ELISA quantification kit (W90-13, Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ specifications.
Khamissi F.Z., Ning L., Kefaloyianni E., Dun H., Arthanarisami A., Keller A., Atkinson J.J., Li W., Wong B., Dietmann S., Lavine K., Kreisel D, & Herrlich A. (2022). Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure. Science Advances, 8(8), eabm5900.
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2

Measuring Albumin Secretion in HepG2 Cells

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HepG2 cells (2 × 105 cells) were cultured in a 60-mm dish, and the culture
medium was changed every 24 h. After 48 h of culture, the cells were incubated in culture
medium for 24 h. The level of albumin secretion was assessed based on the accumulation of
albumin in the culture medium after 24 h using a human albumin enzyme-linked immunosorbent
assay (ELISA) quantification kit in accordance with the manufacturer’s instructions
(Bethyl Laboratories, Inc., Montgomery, TX, USA).
Miyamoto Y., Ikeuchi M., Noguchi H, & Hayashi S. (2018). Long-term Cryopreservation of Human and other Mammalian Cells at −80 °C for 8 Years. Cell Medicine, 10, 2155179017733148.
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3

Differentiation of Liver Buds from PGECs

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Example 9

(Method)

Liver buds were prepared from PGECs (Passage 5, Day 5) using culture plates of low adhesive property. For liver bud preparation, PGEC-derived cells, umbilical vein endothelial cells (HUVEC) and mesenchymal stem cells (MSC) were mixed at a ratio of 10:7:1 and subjected to differentiation and induction in a medium which was a 1:1 mixture of LONZA HCM medium and EGM medium. Albumin concentration in the culture supernatant of PGEC-derived liver buds after differentiation and induction was evaluated with an enzyme-linked immunosorbent assay (ELISA) quantification kit (Bethyl Laboratories Inc.).

(Results)

Microscopic views of PGEC-derived liver buds prepared in vitro are shown in FIG. 26A. Scale bar=50 μm

Albumin secretion ability of PGEC-derived liver buds is shown in FIG. 26B. Human albumin was detected from day 4 of differentiation and induction using HCM/EGM. Further, when compared to tissues from a sphere culture of PGEC alone (i.e., tissues obtained by harvesting PGECs alone, plating at a density of 5×105 cells/well/24-well plate in low-adhesive culture plates with a shape in which cells gather at the bottom and culturing for several days), the PGEC-derived liver buds obviously showed a significantly high albumin secretion ability. When the medium condition was switched from HCM/EGM to KO-DMEM/EGM, albumin secretion was not confirmed.

US10370638B2. Primitive gut endoderm cells and method for producing same (2019-08-06). PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY [JP]. Inventors: Takanori Takebe [JP], Hideki Taniguchi [JP], Ran ran Zhang [JP].
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4

Measuring Sodium Excretion in Mice

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Serum and urine creatinine concentrations were measured with a picric acid-based method (R & D biosystems Minneapolis, MN, USA). Urine albumin concentration was determined by using an ELISA quantification kit, according to the manufacturer’s protocol (Cat# E99-134, Bethyl Laboratories, Inc).
The effect of SKF38393 on sodium excretion was determined by following a previously detailed protocol (23 (link)). Briefly, mice were anesthetized as aforementioned and the right jugular vein was catheterized with PE-10 tubing for infusing saline or SKF38393. A midline abdominal incision was performed, and the urinary bladder was catheterized with PE-50 tubing to collect urine samples. The blood was collected simultaneously through the tail vein. Blood plasma was obtained by centrifuging blood samples at 2000 rpm for 15 minutes at 4 °C. Urine and plasma samples were stored at −80 °C for further analysis. Urine and plasma sodium concentrations were measured by atomic absorption spectroscopy (AAnalyst 400, PerkinElmer, Waltham, MA) to determine urinary sodium excretion (UNaV) and fractional excretion of sodium (FENa).
Farooqui Z., Mohammad R.S., Lokhandwala M.F, & Banday A.A. (2020). Nrf2 Inhibition Induces Oxidative Stress, Renal Inflammation and Hypertension in Mice. Clinical and experimental hypertension (New York, N.Y. : 1993), 43(2), 175-180.
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5

Amino Acid Analysis of Cord Blood

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Cord blood was obtained at cesarean section. For later samples, the femoral vein was accessed percutaneously through ultrasound guidance. Serum and plasma were separated using standard protocols. For amino acid analysis, blood was dried on 903 Protein Saver Cards (GE Healthcare, Pittsburgh, PA). Amino acid and succinylacetone levels were measured by tandem mass spectrometry, as described.21 (link) Cord blood human albumin was measured in triplicate with an enzyme-linked immunosorbent assay (ELISA) quantification kit (Bethyl Laboratories, Montgomery, TX) as per manufacturer's instructions. Plates were read on an EPOCH 2 plate reader with GEN5 (version 2.09) software (BioTek, Winooski, VT).
Nelson E.D., Larson E., Joo D.J., Mao S., Glorioso J., Abu Rmilah A., Zhou W., Jia Y., Mounajjed T., Shi M., Bois M., Wood A., Jin F., Whitworth K., Wells K., Spate A., Samuel M., Minshew A., Walters E., Rinaldo P., Lillegard J.B., Johnson A., Amiot B., Hickey R., Prather R., Platt J.L, & Nyberg S.L. (2022). Limited Expansion of Human Hepatocytes in FAH/RAG2-Deficient Swine. Tissue Engineering. Part A, 28(3-4), 150-160.
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6

Serum IgE Quantification in Mice

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Blood samples were collected by cardiac puncture immediately following euthanasia and serum immunoglobulin E (IgE) concentrations determined with a mouse IgE enzyme-linked immunosorbent assay (ELISA) quantification kit (Bethyl Laboratories, Montgomery, TX, USA) according to the manufacturer's protocol [19 (link)].
Ostrowski A., Nordmeyer D., Mundhenk L., Fluhr J.W., Lademann J., Graf C., Rühl E, & Gruber A.D. (2014). AHAPS-functionalized silica nanoparticles do not modulate allergic contact dermatitis in mice. Nanoscale Research Letters, 9(1), 524.
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7

Isolation and Expansion of hADMSCs

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All human tissues were obtained with approval of the Institutional Review Board of K-Stem Cell (Seoul, Korea) (Protocol No. KS-2015-01-001). hADMSCs were prepared under Good Manufacturing Practice conditions (Biostar Stem Cell Research Institute of R-BIO Co., Ltd., Seoul, Korea). In brief, human abdominal subcutaneous fat tissues were obtained by simple liposuction from a 53-year-old female donor after obtaining informed consent[29 (link)]. The adipose tissues, after digestion with collagenase I, were suspended in Dulbecco’s modified Eagle’s medium (Invitrogen, Grand Island, NY, United States) containing 0.2 mM ascorbic acid and 10% fetal bovine serum (FBS). After overnight culture, the cell medium was changed to Keratinocyte-SFM (serum-free keratinocyte medium; Invitrogen) containing 0.2 mM ascorbic acid, 0.09 mM calcium, 5 ng/mL recombinant epidermal growth factor, and 5% FBS. When the cells reached 90% confluency, they were subculture-expanded in Keratinocyte-SFM medium until passage 3. FBS from cultured stem cells was completely removed by several washes with phosphate-buffered saline (PBS), which was verified by testing the albumin level below the measurement limit using a bovine albumin enzyme-linked immunosorbent assay (ELISA) quantification kit (Bethyl Laboratories, Montgomery, TX, United States).
Shin K., Cha Y., Ban Y.H., Seo D.W., Choi E.K., Park D., Kang S.K., Ra J.C, & Kim Y.B. (2019). Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits. World Journal of Stem Cells, 11(12), 1115-1129.
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8

Breast Milk IL-8 Quantification

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Interleukin (IL)-8 concentrations were measured in breast milk samples by ELISA quantification kits following the manufacturer's instructions (Bethyl, Montgomery, TX and Diaclone, France).
Hurtado J.A., Maldonado-Lobón J.A., Díaz-Ropero M.P., Flores-Rojas K., Uberos J., Leante J.L., Affumicato L., Couce M.L., Garrido J.M., Olivares M, & Fonollá J. (2017). Oral Administration to Nursing Women of Lactobacillus fermentum CECT5716 Prevents Lactational Mastitis Development: A Randomized Controlled Trial. Breastfeeding Medicine, 12(4), 202-209.
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9

Plasma IgA, IgG, Cytokine Quantification

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The total IgA and IgG concentrations in the plasma were measured in duplicates with enzyme-linked immunosorbent assay (ELISA) quantification kits, according to the manufacturer’s instructions (Bethyl, Montgomery, TX, USA).
The cytokine and interleukin concentrations in the plasma were measured in duplicates with ELISA quantitation kits, according to the manufacturer´s instructions (Affymetrix eBioscience, San Diego, CA, USA).
Fonollá J., Gracián C., Maldonado-Lobón J.A., Romero C., Bédmar A., Carrillo J.C., Martín-Castro C., Cabrera A.L., García-Curiel J.M., Rodríguez C., Sanbonmatsu S., Pérez-Ruiz M., Navarro J.M, & Olivares M. (2017). Effects of Lactobacillus coryniformis K8 CECT5711 on the immune response to influenza vaccination and the assessment of common respiratory symptoms in elderly subjects: a randomized controlled trial. European Journal of Nutrition, 58(1), 83-90.
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10

Quantifying Antigen-Specific Antibodies

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Diluted human plasma (1:250 dilution) was added to plates coated with LGL1 (500 ng per well), and the levels of kappa and lambda immunoglobulin light chains were determined with the use of human kappa- and lambda-specific enzyme-linked immunosorbent assay (ELISA) quantification kits (Bethyl Laboratories).15 (link) To measure hen-egg lysozyme (HEL)–specific and lipid (LGL1, DAG, cardiolipin, and lipid A)–specific antibodies, plates (Nunc- Immuno plate, Thermo Scientific) were coated with an equimolar concentration of antigens overnight at room temperature and then blocked with 1% BSA in PBS and Tween 20 detergent for 2 hours at room temperature. The test serum and purified immunoglobulin sample were diluted in 1% BSA in PBS and Tween 20 detergent and incubated overnight at 4°C. The antigen-specific antibody was detected by HRP-conjugated mouse and human immunoglobulins that had been developed with TMB chromogen (3,3′,5,5′-tetramethylbenzidine; Invitrogen).
Nair S., Branagan A.R., Liu J., Boddupalli C.S., Mistry P.K, & Dhodapkar M.V. (2016). Clonal Immunoglobulin against Lysolipids in the Origin of Myeloma. The New England journal of medicine, 374(6), 555-561.
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