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Tnf 210 ta 020

Manufactured by R&D Systems

The TNF (210-TA-020) is a laboratory equipment product offered by R&D Systems. It is designed for the detection and quantification of tumor necrosis factor (TNF) in various sample types.

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Lab products found in correlation

2 protocols using tnf 210 ta 020

1

Signaling Pathways in Chemokine Regulation

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Recombinant human EGF (236-EG-200) and TNF (210-TA-020) were obtained from R&D Systems. Antibodies for IκB (8219), Akt (8200), Erk (8201) and their phosphorylated forms were purchased from Cell Signaling Technology. The PCR array for customized mouse chemokines (CAMP10242) and SYBR® Green Master Mix (330503) came from SABiosciences/Qiagen. Specific PCR primers for chemokines or chemokine receptors were obtained from Eurofins MWG Operon. Chemiluminescent detection kits (sc-2048) were from Santa Cruz Biotechnologies. 3-​Isobutyl-​1-​methylxanthine (IBMX, I-7018), insulin (I-5500), and dexamethasone (D-4902) were purchased from Sigma-Aldrich. All liquid culture media such as FBS (26140) and Dulbecco’s modified Eagle’s medium (DMEM, 11965) were acquired from Invitrogen.
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2

Cytokine-Induced Cell Signaling Assay

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Human recombinant IFN-ɣ (300-02) was obtained from PeproTech, and TNF (210-TA-020) was purchased from R&D systems. (L-)kynurenine (K8625), ouabain octahydrate (O3125), digoxin (D6003), p-dimethylaminobenzaldehyde (D2004), and gramicidin (G5002) were purchased from Sigma Aldrich. SBFI-AM (sc-215841) was purchased from Santa Cruz Biotechnology. Trichloroacetic acid (11462691) and glacial acetic acid (010994-AC) were purchased from Fisher Scientific. Cell culture grade DMSO (A3672.0100) was purchased from AppliChem. Pluronic F-127 (59004) was purchased from Biotium.
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