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Amv first strand synthesis kit

Manufactured by Thermo Fisher Scientific
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The AMV first-strand synthesis kit is a laboratory reagent used for the synthesis of complementary DNA (cDNA) from RNA templates. The kit contains the necessary components, including the AMV (Avian Myeloblastosis Virus) reverse transcriptase enzyme, to facilitate the reverse transcription process, which is a fundamental step in various molecular biology and gene expression analysis workflows.

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Lab products found in correlation

Abi prism 7500 detection system, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions) Taqman primer probe assay, by Thermo Fisher Scientific (1 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Cm3050s cryostat, by Leica (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Glycoblue, by Thermo Fisher Scientific (1 mentions) Lightcycler 96, by Roche (1 mentions) Faststart essential dna green master mix, by Roche (1 mentions) Lightcycler 480 instrument, by Roche (1 mentions)

Close spelling variants of this product

Cloned AMV First-Strand cDNA Synthesis Kit (47 citations) AMV First-Strand cDNA Synthesis Kit (16 citations) Cloned AMV First-Strand Synthesis Kit (11 citations) Clone AMV First-Strand cDNA synthesis kit (4 citations)

3 protocols using amv first strand synthesis kit

1

Quantifying c-fos Expression in Rat PFC

Cited 1 time
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Total RNA was extracted from rat PFC by the phenol–guanidine isothiocyanate–chloroform method as previously described (56 (link)) and further purified using an RNeasy kit with on-column DNase digestion according to the manufacturer’s instructions (Qiagen, Manchester, UK). One microgram of total RNA was converted to complementary DNA using an AMV first-strand synthesis kit (Invitrogen) and c-fos expression quantified by quantitative reverse transcription polymerase chain reaction using a TaqMan primer-probe assay (no. Rn02396759_m1; Applied Biosystems, Warrington, UK) on an ABI Prism 7500 detection system (Applied Biosystems). Data were normalized to the β-actin housekeeping gene (assay ID 4352931E; Applied Biosystems), and relative expression was analyzed by the ΔΔCt method.
Pooley J.R., Flynn B.P., Grøntved L., Baek S., Guertin M.J., Kershaw Y.M., Birnie M.T., Pellatt A., Rivers C.A., Schiltz R.L., Hager G.L., Lightman S.L, & Conway-Campbell B.L. (2017). Genome-Wide Identification of Basic Helix–Loop–Helix and NF-1 Motifs Underlying GR Binding Sites in Male Rat Hippocampus. Endocrinology, 158(5), 1486-1501.
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2

Quantitative PCR analysis of vlPAG

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At the end of each experiment, brains were removed, flash frozen, and sectioned at 300 μm with a Leica CM3050S cryostat and mounted on to sterile slides. One-millimeter bilateral micropunches were taken from 6 levels of the vlPAG (Bregma −6.72, −7.04, −7.64, −8.0, −8.30, −8.80) and RNA was extracted with TRIzol (Life Technologies; 15596026) using standard procedures, followed by the addition of Glycoblue (Life Technologies; AM5916) for visualization. Concentrations of RNA (ng/μl) were calculated using a NanoDrop ND-1000 Spectrophotometer (Version 3.8, Thermo Fisher; DE). Following RNA extraction, RNA was diluted to a standard concentration and converted to cDNA using an AMV First-Strand Synthesis Kit (Invitrogen). PCR was performed using FastStart Essential DNA Green MasterMix (Roche) and analyzed using a Roche LightCycler 96 and accompanying software (Version 1.1.0.1320, 2011 Roche Diagnostics; Switzerland). Primer sequences are provided in Table 1.
Doyle H.H, & Murphy A.Z. (2017). Sex-Dependent Influences of Morphine and its Metabolites on Pain Sensitivity in the Rat. Physiology & behavior, 187, 32-41.
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3

qRT-PCR Analysis of Hoxa5 Expression

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For qRT-PCR on neck/trunk segments from the Hoxa5 expression domain, C3-T2 segments were dissected from embryos of the appropriate ages, and forelimb tissue, neural tube, and thoracic organs were removed. RNA extraction was performed by Trizol method, cDNA synthesis was performed with the AMV first strand synthesis kit (Invitrogen), and qPCR with the Applied Biosystems Sybr Master I mix, all following manufacturer’s instructions. qRT-PCR was performed on a Roche Lightcycler 480 instrument. Primer sequences are given in Supplementary Table 2.
Holzman M.A., Ryckman A., Finkelstein T.M., Landry-Truchon K., Schindler K.A., Bergmann J.M., Jeannotte L, & Mansfield J.H. (2021). HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Frontiers in Cell and Developmental Biology, 9, 632303.
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