To measure biochemical parameters and complete blood counts, blood was collected in a serum-separating tube gel and clot activator tube (Becton Dickinson, Franklin Lakes, NJ, USA). Serum was separated by centrifugation (1,500 g, 10 min at room temperature). An automated chemistry analyzer (Hitachi-747; Hitachi Medical, Tokyo, Japan) was used to measure the serum levels of triglyceride (TG), glucose (GLU), total protein (T-PRO), albumin (ALB), blood urea nitrogen (BUN), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). A part of the blood was collected in a test tube containing ethylenediaminetetraacetic acid (Vacutainer K3E, BD Biosciences, Plymouth, UK) to measure the blood cell count using an automated blood cell analyzer (Sysmex NE-8000, TOA Medical Electronics, Kobe, Japan).
Vacutainer k3e
Manufactured by BD
Sourced in United Kingdom
The Vacutainer K3E is a blood collection tube designed for the collection of venous blood samples. It contains the anticoagulant K3EDTA, which prevents blood from clotting during the collection and transportation process. The tube is used to collect blood samples for various laboratory tests, such as complete blood counts, hemoglobin, and hematocrit measurements.
Automatically generated - may contain errors
11 protocols using vacutainer k3e
1
Comprehensive Blood Analysis Protocol
2
Blood Chemistry Analysis Protocol
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To measure the blood chemistry parameters, blood samples were collected from the abdominal vein in a SST® gel & clot activator tube (Becton and Dickinson, Franklin Lakes, NJ, USA). Serum was separated by centrifugation at 1500 × g for 10 min at room temperature. An automated chemistry analyzer (Hitachi-747, Hitachi Medical Co., Tokyo, Japan) was used to measure serum biochemical parameters association with lipid metabolism and liver functions including the aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyl transpeptidase (γ-GTP), albumin (Alb), total cholesterol (T-Cho), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C). A part of blood was collected in a test tube containing ethylenediaminetetraacetic acid (EDTA) (Vacutainer K3E, BD Biosciences, Plymouth, UK) as an anticoagulant to measure the number of blood cell and analyzed using an automated blood cell analyzer (Sysmex NE-8000, TOA Medical Electronics, Kobe, Japan).
3
Assessing Blood Microbial Diversity
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Three ml of blood was collected from seven clinically healthy adults in Vacutainer tubes with heparin and K3EDTA as anticoagulants (Vacutainer K3E and Vacutainer Heparin Tube, BD, USA). All samples were previously characterized for their microbial biodiversity content by applying 16S rDNA and ITS targeted next-generation sequencing (Panaiotov et al., 2021 (link)). Two hundred µl of all blood samples were tested for sterility by growing on Sabouraud and blood agar 90 mm plates at 37°C for 72 hours.
4
Sepsis Biomarkers from Intensive Care
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71 blood samples were taken from patients with clinical symptoms of sepsis, hospitalized in the John Paul II Hospital in Krakow at The Ward of Anesthesiology and Intensive Care. Blood samples were drawn into 4-ml Vacutainer K3E (BectonDickinson) test tubes. Patients were enrolled into the study according to the SIRS criteria [23 ].
5
Sepsis in Cardiac Surgery Patients
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Eighty five blood samples were included in this study. Sixty two were from patients hospitalized in the John Paul II Hospital in Krakow at The Ward of Anesthesiology and Intensive Care. They underwent serious cardiac surgical procedures and had clinical symptoms of sepsis according to criteria of the Society of Critical Care Medicine (SCCM) and The European Society of Intensive Care Medicine (ESICM) [12 (link)]. The median age of these patients was 67 years. Among them 14 were female and 48 were male. Twenty three blood samples were collected from healthy volunteers who had no clinical symptoms of sepsis and no elevated level of inflammatory markers (CRP, OB). The median age of healthy subjects was 59 years. Among them 13 were female and 10 were male. No patient or volunteer was treated with antibiotics before the collection of blood samples. Blood samples were drawn into 4-ml Vacutainer K3E (BectonDickinson) test tubes.
6
Sepsis Biomarkers in Clinical Samples
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Blood was collected from volunteers, who had no clinical symptoms of sepsis and no inflammatory markers (CRP, OB). Additionally, 102 blood samples were taken from patients with clinical symptoms of sepsis, hospitalized in the John Paul II Hospital in Krakow. Blood samples were drawn into 2-ml Vacutainer K3E (BectonDickinson) test tubes.
7
Biospecimen Collection: RCC Patients
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Urine and plasma samples were collected from patients affected by Renal Cell Carcinoma (RCC) the day before surgery at San Gerardo Hospital (Monza, Italy). All subjects had signed an informed consent prior to sample donation and analyses were carried out in agreement with the Declaration of Helsinki. Study protocols and procedures were approved by the local ethic committee (Comitato Etico Azienda Ospedaliera San Gerardo, Monza, Italy). Second morning midstream urine was collected in sterile urine tubes (Anicrin s.r.l., Italy). After centrifugation at 3000 rpm for 10 min, samples were kept at -80°C [16] (link).
Plasma samples were collected in Vacutainer® K3E containing EDTA (Becton Dickinson Italia S.p.A.), centrifuged at 3700rpm for 10 minutes and then stocked at -80°C.
Plasma samples were collected in Vacutainer® K3E containing EDTA (Becton Dickinson Italia S.p.A.), centrifuged at 3700rpm for 10 minutes and then stocked at -80°C.
8
Bacteriological Investigation Protocols
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Bacteriological investigations were done using standard bacteriological procedures (Quinn et al. 1994) . MacConkey and blood agar were used for cultivating of samples. For preparing of blood agar to Columbia agar base 10% (v/v) bovine anticoagulated blood in 10 ml Vacutainer K3E (Becton Dickinson) tubes were added. Biochemical characterization and identification of isolated bacterial strains were performed with the "Crystal" indication system (Becton Dickinson, USA).
9
Quantification of RANGAP1 Protein in Blood Samples
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Blood samples were obtained using peripheral venipuncture via a 10 mL glass vacuum extraction tube, treated with 15% EDTA anticoagulant (0.12 mL) (BD Vacutainer® K3E; REF 368480). The tubes were centrifuged (Eppendorf 5415R, Eppendorf, Germany) at 1300 rpm for 10 min at 4 °C, and the supernatant was collected and aliquoted into 500 μL screen-printed plastic cryotubes, which were subsequently stored in the biobank of La Fe University Hospital (Valencia, Spain) at −80 °C until further analysis. The assay was performed on all samples at the same time.
RANGAP1 expression was determined using a specific ELISA kit (cat. MBS9321016; MyBiosurce Inc., San Diego, CA, USA). The RANGAP1 test has a detection limit of 1.00 ng/mL and sensitivity of 1.00 ng/mL; both intra-assay CV (%) and inter-assay CV (%) are less than 15%.
RANGAP1 expression was determined using a specific ELISA kit (cat. MBS9321016; MyBiosurce Inc., San Diego, CA, USA). The RANGAP1 test has a detection limit of 1.00 ng/mL and sensitivity of 1.00 ng/mL; both intra-assay CV (%) and inter-assay CV (%) are less than 15%.
10
Quantifying Plasma Hormones in Rats
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Venous blood was transferred into plasma vials (BD Vacutainer K3E with 250 KIU aprotinin), left on ice for 30 min and centrifuged for 10 min at 2000 g. The supernatant (plasma) was stored at –80 °C. Quantitative measurement of insulin and glucagon levels was performed using a rat insulin (#SRI-13K) or glucagon (#GLK-32) RIA (Merck (Millipore), St. Charles, Missouri, USA) according to the manufacturer's instructions. Insulin levels were also measured in serum, which was generated without aprotinin and EDTA, using a rat insulin ELISA (DRG Instruments GmbH, Marburg, Germany). Leptin and adiponectin levels were determined using ELISA (Leptin Quantikine Elisa Mouse / Rat Immunoassay, Cat.-Nr. MOB00, R&D Systems, Wiesbaden, Germany; Adiponectin (ADP) Elisa Mouse / Rat / Human, Cat.-Nr. MBS066180, MyBioSource, USA).
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