Log In Sign up
The largest database of trusted experimental protocols

Fluorescence microscopy system

Manufactured by Olympus
Visit Supplier
Sourced in Japan

The Fluorescence Microscopy System is a scientific instrument designed for the observation and analysis of fluorescently labeled samples. The system uses a light source, optical filters, and a sensitive camera to capture images of the fluorescent signals emitted by the sample. This allows researchers to visualize and study the distribution and behavior of specific molecules or structures within cells or tissues.

Automatically generated - may contain errors

Lab products found in correlation

All in one fluorescence microscope bzx800e system, by Keyence (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Sc 3598, by Santa Cruz Biotechnology (1 mentions) Lsm 780 confocal microscope, by Zeiss (1 mentions) Paraformaldehyde solution, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Mettl3 e3f2a rabbit mab, by Cell Signaling Technology (1 mentions) Cd68 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

BX51 fluorescence microscopy system Reflected fluorescence microscopy system Digital light/fluorescence microscopy system Inverted fluorescence microscopy system Epi-fluorescence microscopy system IX71 fluorescence microscopy system Fluorescence microscopy

4 protocols using fluorescence microscopy system

1

Immunofluorescence Imaging of Parasite Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parasites were allowed to invade HEK293T cells on coverslips at given MOIs and incubated for indicated time points. Next, cells were fixed with 4% formaldehyde for 20 min at room temperature. For parasite invasion assay, the extracellular parasites were stained with mouse anti-SAG1 antibody first and then washed three times using PBS. Then, cells were permeabilized and blocked by treatment with 0.1% Triton X-100 and 5% FBS. Subsequently, the coverslips were incubated with rabbit anti-ALD antibody and then with the respective secondary plus Hoechst (goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488; Invitrogen, USA). For detection of p65 nuclear translocation and expression of recombinant or truncated GRA15 proteins, coverslips of transfected cells were incubated with primary antibodies (mouse anti-FLAG and rabbit anti-p65; CST, USA) at indicated time points. Images were acquired using Olympus fluorescence microscopy system.
Guo G., Cui J., Song L., Tang L., Fan S., Shen B., Fang R., Hu M., Zhao J, & Zhou Y. (2022). Activation of NF-κB signaling by the dense granule protein GRA15 of a newly isolated type 1 Toxoplasma gondii strain. Parasites & Vectors, 15, 347.
+ Open protocol
+ Expand
2

Synovial Tissue Quality Monitoring and Multiplex Immunohistochemistry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue quality was monitored by staining four-μm thick sections of all synovial tissue samples with Hematoxylin & Eosin Stain Kits (Vector laboratories, H-3502). Immunohistochemical staining was performed as in previous reports25 (link). Briefly, four-μm thick sections of synovial biopsies were permeabilized with 0.5% Triton-X-100 in PBS (20 min). For multiplex immunohistochemistry staining, tissue sections were incubated with primary anti-CD68 and anti-CCL18 antibodies, anti-human CD3 and anti-IFNγ at 4°C overnight. Subsequently, appropriate secondary antibodies were added at 37°C for 1h. The primary and secondary antibodies are listed in Supplemental Table 4. Nuclei were marked with DAPI (1:2,000, SC-3598, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 min at room temperature. Immunofluorescence images were acquired using an Olympus fluorescence microscopy system (Olympus, Tokyo, Japan) or the Keyence BZX800E system (Keyence, Kyoto, Japan).
Hu Z., Zhao T.V., Huang T., Ohtsuki S., Jin K., Goronzy I.N., Wu B., Abdel M.P., Bettencourt J.W., Berry G.J., Goronzy J.J, & Weyand C.M. (2022). The transcription factor RFX5 coordinates antigen-presenting function and resistance to nutrient stress in synovial macrophages. Nature metabolism, 4(6), 759-774.
+ Open protocol
+ Expand
3

Immunohistochemistry for CD96 and CD155

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were shock-frozen in OCT on dry ice and blocks stored in −80°C as described.5 (link),62 (link) Five-micron sections were fixed with pre-cold acetone for 15 min and incubated with 0.3% H2O2 buffer for 15 min at room temperature. For dual-color immunohistochemistry, tissue sections were stained with primary antibodies. Bound antibodies were visualized with anti-rabbit or anti-mouse secondary antibodies. Tissues were counterstained with DAPI (#D1306, Thermo Fisher Scientific) and images were taken with an Olympus fluorescence microscopy system (Olympus, Tokyo, Japan). Tissues stained with anti-CD96 or anti-CD155 antibodies were analyzed by the All-in-One Fluorescence Microscope BZX800E system (Keyence, Kyoto, Japan) and Zeiss LSM780 confocal microscope (Zeiss, Oberkochen, Germany). The antibodies used in immunohistochemistry are listed in Table S2.
Ohtsuki S., Wang C., Watanabe R., Zhang H., Akiyama M., Bois M.C., Maleszewski J.J., Warrington K.J., Berry G.J., Goronzy J.J, & Weyand C.M. (2023). Deficiency of the CD155-CD96 immune checkpoint controls IL-9 production in giant cell arteritis. Cell Reports Medicine, 4(4), 101012.
+ Open protocol
+ Expand
4

Dual-Color Immunostaining for Atherosclerotic Plaques

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The methods used for dual-color immunostaining have previously been described29 (link). Cells were fixed with 4% paraformaldehyde solution (Affymetrix) in glass bottom tissue culture plates, incubated with primary antibody at 4 °C overnight, followed by fluorescence conjugated secondary antibody at room temperature for 2 hr. For tissue staining, atherosclerotic plaques were cut into 4μm thick sections and permeabilized with 0.5% Triton-X-100 in PBS for 20 min. Tissue sections were incubated with primary antibodies for overnight at 4 °C and secondary antibodies for 1 hour at 37°C. Nuclei were labeled with DAPI (Santa Cruz Biotechnology) for 10 mins at room temperature. Images were analyzed using the Olympus fluorescence microscopy system (Olympus, Tokyo, Japan) or the All-in-One Fluorescence Microscope BZX800E system (Keyence, Kyoto, Japan). Following antibodies were used: CD155 Monoclonal Antibody (Thermo Fisher Scientific, MA5–13493,1:200), CD68 Monoclonal Antibody (Thermo Fisher Scientific,MA5–13324,1:200), METTL3 (E3F2A) Rabbit mAb (Cell Signaling Technology 86132S,1:200), Goat anti-rabbit IgG (H+L), Alexa Fluor 488 (Thermo Fisher Scientific, A-11008,1:200) and Goat anti-mouse IgG (H+L), Alexa Fluor 594 (Thermo Fisher Scientific, A-31635,1:200). Detailed information of all antibodies used are listed in Supplementary Table 2.
Zhao T.V., Hu Z., Ohtsuki S., Jin K., Wu B., Berry G.J., Frye R.L., Goronzy J.J, & Weyand C.M. (2022). Hyperactivity of the CD155 immune checkpoint suppresses anti-viral immunity in patients with coronary artery disease. Nature cardiovascular research, 1(7), 634-648.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!