For in vivo assays, serum samples were collected at 0, 12, 30, and 60 h after CLP. For in vitro assays, culture medium supernatants were collected at 0, 6, 12, and 18 h after LPS or HMGB1 administration. The levels of HMGB1 (LSBio, Seattle, WA, USA), TNF-α, IL-1β, and IL-6 were determined using enzyme linked immunosorbent assay (ELISA) kits (all from R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
Hmgb1
Manufactured by LSBio
Sourced in United States
HMGB1 is a protein that acts as a chromatin-binding factor and participates in the regulation of gene transcription. It is involved in various cellular processes, including DNA repair, inflammation, and cell differentiation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using hmgb1
1
HMGB1 and Inflammatory Cytokine Kinetics
2
Serum Biomarker Analysis in Mice
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Mice were anesthetized with 1.5–2% isoflurane and blood was collected by intra-cardiac puncture. Serum was isolated from blood after 30-min room temperature incubation and centrifugation. Aspartate transaminase activity (BioAssay Systems), HMGB1 (LSBio), iron (Abcam), hepcidin (eLabScience), NGAL (ENZO Life Sciences), hemopexin (Alpha Diagnostic), haptoglobin (Alpha Diagnostic), and ferritin (Kamiya Biomed) were measured in the serum according to manufacturers' protocols.
3
Murine Cytokine Quantification via ELISA
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The release of murine TNFα, CXCL1, CXCL2/3, IL-33 (all R&D Systems), and HMGB1 (E-20749; Qayee-Bio) in the BAL of mice after LPS inhalation was determined by enzyme-linked immunosorbent assay kits according to the manufacturer's instructions.
Additionally, we detected IL-33 and HMGB1 from the supernatants of bone marrow neutrophils under the indicated conditions. Human IL-33 (R&D Systems) and HMGB1 (LS-F4038-1; LS-Bio) were measured from the supernatants of human PMNs. Western blots intensity was measured by ImageJ (Version 1.49v; National Institute of Health; USA).
Additionally, we detected IL-33 and HMGB1 from the supernatants of bone marrow neutrophils under the indicated conditions. Human IL-33 (R&D Systems) and HMGB1 (LS-F4038-1; LS-Bio) were measured from the supernatants of human PMNs. Western blots intensity was measured by ImageJ (Version 1.49v; National Institute of Health; USA).
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