Nicolet istm 10 ftir spectrometer

UV–visible spectral analysis was performed by detecting of the optical density (OD) using a “T80” UV/VIS spectrometer (Bruker Corporation, Billerica, MA, USA). Measurements were performed at room temperature between 200 and 800 nm ranges. The baseline was established by using silver nitrate (1 mM) as a blank. Transmission electron microscopy (JEOL JEM-2100 instrument, (JEOL Ltd., Tokyo, Japan)) was utilized to explore the morphology and size of silver nanoparticles. The sample was equipped by bringing a drop of them on a carbon-coated copper grid and using a lamp to dry it.
Fourier transform infrared (FTIR) spectroscopy measurements were used to confirm the AgNPs synthesis and also to estimate the possible bioactive components in the plant latex that enhance the reduction of the Ag⁺ ions and play roles in stabilization of the synthesized nanoparticles [122 (link)]. Both crude latex and silver nanoparticle samples were ground to dry semisolid form and mixed with Kbr and analyzed using a Nicolet^TM iS^TM 10 FTIR spectrometer (Thermo Scientific, Inc., Waltham, MA, USA). The results were detected in the range of 4000–400 cm⁻¹ at a resolution of 8 cm⁻¹ at 25°C.

FT-IR was performed to purified QDs. Samples were dried using a vacuum centrifugal concentrator (IR Concentrator Micro-Cenvac NB-503CIR, N-Biotek Inc.) for 24 h. Then FT-IR spectra were determined using a Nicolet^TM iSTM10 FT-IR Spectrometer (Thermo Scientific Inc.) with a Smart iTRTM Attenuated Total Reflectance (ATR) accessory provided with a single bounce Ge crystal. The scan frequency was 4000 to 700 cm⁻¹.