Nirvana 640st

A home-built imaging system was used for the mouse whole body imaging and paraffin-embedded tissues as described previously^{18 (link)}. A lamp (IP-307TCS, Lanics; 800-nm short pass filter, FIT Leadintex, Inc., Tokyo, Japan) was placed obliquely above the mouse, and the NIR camera (InGaAs-array video camera, NIRvana 640ST, Princeton Instruments, Trenton, NJ, USA), above the mouse. An objective lens (Cosmicar, Pentax, RICOH Imaging Company, Ltd., Tokyo, Japan) and 1000 nm long-pass filter were used to exclude lights other than CNT fluorescence. The NIR camera exposure time was 100 ms.

For angle-resolved reflectivity measurements, the emission of a calibrated lamp was focused onto the sample by an NIR corrected × 100 objective with 0.8 NA (Olympus LMPL100xIR). The resulting spot diameter of ∼2 μm defines the investigated area on the sample. For PL measurements, the white light source was replaced by a laser diode (OBIS, Coherent Inc., 640 nm, cw, ∼10 mW). The light reflected/emitted by the cavity was imaged onto the entrance slit of a spectrometer (Princeton Instruments IsoPlane) using a 4f Fourier imaging system and was recorded by a 640 × 512 InGaAs camera (Princeton Instruments, NIRvana:640ST). Additionally, a long-pass filter (cut-off wavelength, 850 nm) and a linear polarizer were placed in front of the spectrometer. Angle calibration of the system was done by fitting the measured cavity mode of a reference sample of known refractive index.

Cells were harvested during the mid-exponential growth phase (OD_750nm between 1 and 1.5), pelleted by centrifugation, washed twice with 1 mM HEPES buffer (pH 7.4), and re-suspended in the same buffer to obtain an OD_750nm = 0.9. Cells were fixed onto poly-L-lysine coated glass-bottom petri dishes by spotting 30 µL of the cell–SWCNT suspensions for 10 min, followed by washing with 1 mM HEPES buffer (pH 7.4). Fixed cells were treated with 50 µL LSZ–SWCNTs (concentration of 2 mg/L) and incubated at room temperature for 10 min in the dark prior to washing with 1 mM HEPES buffer (pH 7.4).
Cells were imaged using a custom-built optical setup consisting of an inverted microscope (Eclipse Ti-E, Nikon AG Instruments) with an oil-immersion TIRF Apo 100 × objective (N.A. 1.49, Nikon) coupled to a CREST X-Light spinning-disk confocal imaging system (CREST Optics) (60 µm pinholes) and an InGaAs camera (NIRvana 640 ST, Princeton Instruments). The setup has an axial resolution of 0.6 ± 0.1 μm and a lateral resolution 0.5 ± 0.1 μm. Samples were illuminated using a TriLine LaserBank system (Cairn Research) at 640 nm and 780 nm, and fluorescence was collected using either an 800 nm (Chroma) or a 980 nm long-pass filter (Semrock). Images were acquired using the Nikon NIS-Elements software (Nikon Instruments).