Micromass quattro micro triple quadrupole

Samples were also analysed by an HPLC-DAD-MS/MS system: a Waters Alliance 2695 (Waters^®, Dublin, Ireland) separation module with an autosampler (20 μL injection volume), a quaternary pump and a solvent degasser, coupled to a Photodiode Array Detector Waters 996 PDA (Waters, Dublin, Ireland) scanning wavelength absorption between 210 and 600 nm. A LiChrospher^® 100 RP-18 5 μm column at 35 °C (stabilized by a column oven) was used. Tandem mass spectrometry (MS/MS) detection was carried out with a Micromass^® Quattro Micro triple quadrupole (Waters, Dublin, Ireland), using an electrospray ionization source in both positive (ESI+) and negative (ESI-) modes. A full scan mode (m/z: 60–1100) record was applied for the mass spectra of the compounds separated by HPLC, using a collision energy of 20 eV. The HPLC gradient method and eluents are described in ‘Supplementary Materials Table S2’ section. The MS/MS conditions, as source temperature, capillary and source voltages have been previously described by Katsinas et al. [16 (link)]. For data acquisition and processing, MassLynx^® 4.1 software (Waters, Dublin, Ireland) was used.

The phenolic compounds present in the freshly prepared aqueous solutions of CONV and OPT3, and the degradation phenolic products present in the 40 °C exposed aqueous solutions of OL, HT, CONV, and OPT3, were putatively identified by an HPLC-DAD-MS/MS system: Waters Alliance 2695 (Waters^®, Dublin, Dublin, Ireland) separation module with an autosampler (10 μL injection volume), a quaternary pump and a solvent degasser, coupled to a Photodiode Array Detector Waters 996 PDA (Waters^®, Dublin, Ireland) scanning wavelength absorption between 210 and 600 nm. A LiChrospher^® 100 RP-18 5 μm (250 × 4.0 mm) (Sigma-Aldrich, St. Louis, MO, USA) column at 35 °C (stabilized by a column oven) was used. MS/MS detection was carried out with a Micromass^® Quattro Micro triple quadrupole (Waters^®, Dublin, Ireland), using an ESI. A full scan mode (m/z: 60–1100) record was applied for the mass spectra of the compounds separated by HPLC, using a collision energy of 20 eV. The HPLC gradient method, eluents, and elution program used, together with the source temperature, capillary, and source voltages are described by Katsinas et al. [16 (link)] For data acquisition and processing, MassLynx^® 4.1 software (Waters^®, Dublin, Ireland) was used.

Samples were analyzed using a Waters Alliance 2695 (Waters^®, Dublin, Ireland) equipped with a quaternary pump, solvent degasser, auto sampler and column oven, coupled to a Photodiode Array Detector Waters 996 PDA and a Micromass^® Quattro Micro triple quadrupole (Waters^®, Dublin, Ireland). The separation of the compounds was performed on a reversed-phase column (LiCrospher^® 100 RP-18, 250 × 4 mm; 5 µm; Merck^®, Rahway, NJ, USA) at 35 °C, using an injection volume of 20 µL. The mobile phase consisted of Milli-Q water containing 0.1% formic acid (A): acetonitrile (B) at a flow rate of 0.30 mL/min, following the method described in Bento-Silva et al., 2020. DAD was used to scan wavelength absorption from 210 to 600 nm. Tandem mass spectrometry (MS/MS) detection was performed using an electrospray ionization (ESI) source operating at 120 °C, applying the conditions previously described (Bento-Silva et al., 2020) [15 (link)].