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5 protocols using sodium tungstate dihydrate

1

Metal Chloride Catalyzed Oxidation Assay

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Iron(II) chloride tetrahydrate (FeCl2·4H2O), cobalt chloride hexahydrate (CoCl2·6H2O), manganese chloride tetrahydrate (MnCl2·4H2O), nickel chloride hexahydrate (NiCl2·6H2O), Sodium tungstate dihydrate (Na2WO4·2H2O), hexadecyl trimethyl ammonium Bromide (CTAB), 1,3-diphenylisobenzofuran (DPBF) and 3,3,5,5-Tetramethylbenzidine (TMB) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All chemicals were of analytical grade and used without further purification.
The ROS detection kit (S0033S) and propidium iodide (PI) (ST511) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The primary antibodies, Anti-GAPDH and anti-AID, were purchased from Abways Technology Co., Ltd. (Shanghai, China). and North American ImmunoWay Biotechnology Company (TX, USA). The ECL Substrate Kit (P0018FS) was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China).
Human DLBCL SU-DHL-4 and OCI-LY19 cell lines were purchased from BeNa Culture Collection (BeNa, #BNCC340176, #BNCC338225) (Suzhou, China).
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2

Tungstate-based Colorimetric Assay

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A reagent grade sodium tungstate dihydrate (Na2WO4·2H2O, 99.5%), citric acid (C6H8O7·H2O, 99.5%), oxalic acid (C2H2O4·2H2O, 99.5%), tartaric acid (C4H6O6, 95%, Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), hydrochloric acid (HCl, 37%, Da Mao Chemical Reagent Factory, Tianjin, China) and ethanol (C2H5OH, 99.5%) were used directly without further purification. All reagents except those indicated are from Sinopharm Chemical Reagent Co., Ltd., (Shanghai, China).
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3

Synthesis of Tungstate Nanostructures

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Sodium tungstate dihydrate (Na2WO4·2H2O, 99.5%), nitric acid (HNO3, 65–68%), and tetramethylammonium hydroxide (TMAOH, 96%) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China), Damao Chemical Reagent Factory (Tianjin, China), and Dubai Biological Technology Co., Ltd. (Shanghai, China), respectively. The above three chemical reagents were used as received without further purification.
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4

Synthesis of Tungsten Oxide Nanoparticles

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All the chemicals used in the experiments were analytical reagents (AR). Sodium tungstate dihydrate (Na2WO4·2H2O) was purchased from Macklin. Concentrated hydrochloric acid (HCl, 36%) and hydrogen peroxide (30%) were purchased from the Zhengzhou Paini Chemicals Reagent Factory.
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5

Synthesis of Bismuth-Tungstate Photocatalysts

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Sodium tungstate dihydrate (Na2WO4·2H2O, 99.5%) and titanium dioxide (P25) were purchased from Shanghai Macklin Biochemical Co., Ltd, China. Bismuth(III) nitrate pentahydrate (Bi(NO3)3·5H2O, 99.0%) was purchased from Sinopharm Chemical Reagent Co., Ltd, China. Cetyltrimethylammonium bromide (CTAB, 99.0%) was purchased from Damao Chemical Reagent Factory Co., Ltd, China. Sodium hydrogen carbonate (NaHCO3, 99.5%) was purchased from Guangdong Guanghua Sci-Tech Co., Ltd, China. All chemicals were used as received without further purification. The water used throughout the research is deionized water.
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