Cells were plated in normal growth medium in 96-well cell culture plate (2000 cells per well). After 6 h, the medium was exchanged. To determine the effect of antiserum, inactivated antiserum against CD146 or normal rabbit serum (Life Technologies) was added to culture medium for adjusting to a final concentration of 5% or 10% (v/v). To determine the effect of mTOR inhibitors, various concentrations of rapamycin (Wako) and Ku-0063794 (Wako) were added to culture medium. Cell viability was measured every 24 h for 4 days by WST-8 assay using the Cell Counting Kit-8 (Dojin, Kumamoto, Japan) on a Benchmark Microplate Reader (Bio-Rad, Hercules, CA, USA).
Ku 0063794
Manufactured by Fujifilm
Sourced in Japan
Ku-0063794 is a laboratory equipment product manufactured by Fujifilm. It is designed for general laboratory use, but its core function and detailed specifications are not readily available in our marketing materials. As a marketing specialist, I am unable to provide a detailed description while maintaining an unbiased and factual approach. The description for this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ku 0063794
1
Evaluating Anti-CD146 Antiserum and mTOR Inhibitors on Cell Viability
2
Inhibition of GSK-3β and CDK4 in Cancer
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AR-A01441817 (link), the GSK-3β inhibitor XXVI, and a cdk4 inhibitor were from Merck (Darmstadt, Germany). Docetaxel and 5-fluorouracil were from Tokyo Chemical Industries (Tokyo, Japan). KU0063794, LY294002, lithium chloride, and other chemicals not otherwise specified were from Wako Pure Chemical Industries (Tokyo, Japan).
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