Sc 15393 x

Manufactured by Santa Cruz Biotechnology

Sourced in United States

SC-15393 X is a laboratory instrument designed for cell culture applications. It is a compact and benchtop-sized incubator that maintains a controlled environment for cell growth and proliferation. The product specifications and technical details are available upon request.

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Lab products found in correlation

Ab171870, by Abcam (2 mentions) Sybr green based detection method, by Thermo Fisher Scientific (1 mentions) Protein a agarose beads, by GE Healthcare (1 mentions)

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Sc-15393 (6 citations)

2 protocols using sc 15393 x

Twist-1 Chromatin Immunoprecipitation Assay

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Chromatin immunoprecipitation (ChIP) assay was performed as previously described16 (link). Real-time PCR was conducted by SYBR Green-based detection method (Applied Biosystems) using equal amounts of ChIP and diluted input DNAs. Antibodies used for the ChIP assays included anti-Twist-1 (SC-15393 X, Santa Cruz, USA) and irrelevant IgG control antibody (AB171870, Abcam, UK).

Zhao N., Sun H., Sun B., Zhu D., Zhao X., Wang Y., Gu Q., Dong X., Liu F., Zhang Y, & Li X. (2016). miR-27a-3p suppresses tumor metastasis and VM by down-regulating VE-cadherin expression and inhibiting EMT: an essential role for Twist-1 in HCC. Scientific Reports, 6, 23091.

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Twist1 Chromatin Immunoprecipitation Assay

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Detailed procedures for ChIP assay were described in detail in the Supplementaryary materials and methods. Antibodies against Twist1 (SC-15393 X, Santa Cruz, USA), IgG (AB171870, Abcam, UK) were used in this experiment (5 μg). Antibody-protein-DNA complexes were isolated by immunoprecipitation with protein A agarose beads (GE Healthcare, UK). Following extensive washing, bound DNA fragments were eluted and analyzed by subsequent qRT-PCR. Primer sequences are listed in the Table S1.

Wang Y., Sun B., Zhao X., Zhao N., Sun R., Zhu D., Zhang Y., Li Y., Gu Q., Dong X., Wang M, & An J. (2016). Twist1-related miR-26b-5p suppresses epithelial-mesenchymal transition, migration and invasion by targeting SMAD1 in hepatocellular carcinoma. Oncotarget, 7(17), 24383-24401.

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