S302c thermal sensor head

The light source used is a mounted LED at 405 nm wavelength (M405L4) [8 (link)]. An aspheric condenser lens, Ø1”, f = 16 mm, NA=0.79, ARC: 350–700 nm, was attached to the mounted LED using an SM1 Lens Tube, 1.00” thread depth to collimate the light beam to illuminate an area with a diameter of 2 cm, sufficient to illuminate four wells at time. A T-Cube LED Driver, 1200 mA Max Drive Current (LEDD1B) was used to operate the mounted LED and to control the output power. The output power was monitored using a PM100USB power meter connected to a S302C thermal sensor head, all purchased from Thorlabs (Newton, NJ, USA).

The light source used for the photoactivation process is a mounted blue LED at 405 nm (M405L4) [22 (link)]. The light beam was collimated using an aspheric condenser lens attached to the mounted LED using an SM1 Lens Tube to illuminate an area of 1 cm. A T-Cube LED Driver, 1200 mA Max Drive Current (LEDD1B) was used to control the output power. The output power was monitored using a PM100USB power meter connected to a S302C thermal sensor head, all purchased from Thorlabs (Newton, NJ, USA).