Polyethylene glycol 3350 peg 3350

Streptomyces sp. RM-5-8 was isolated from the Ruth Mullins underground coal mine fire site and was provided by the University of Kentucky CPRI Natural Products Repository. All primers were purchased from Integrated DNA Technology, E. coli 5α and BL21(DE3) competent cells were purchased from New England Biolabs. Dimethylallyl S-thiolodiphosphate (DMSPP) was purchased from Echelon Biosciences. Polyethylene glycol 3350 (PEG 3350) and PEG 4000 both in the form of a 50% w/v solution, as well as crystallization screens Index HT, PEGRx HT, Crystal Screen HT and SaltRx HT were obtained from Hampton Research. Crystallization screens JCSG-plus HT-96, Morpheus and MIDAS were from Molecular Dimensions. All other reagents and chemicals were purchased from Sigma-Aldrich or Fisher Scientific and were used without further purification unless otherwise stated. PD-10 columns and Ni-NTA superflow columns were purchased from GE Healthcare. All non-native synthetic prenyl donors were synthesized as previously reported^{26 (link),27 (link)}. All solvents used were of ACS grade and purchased from Pharmco-AAPER. All DNA sequencing was conducted with the primers T7 promoter (5′-TAATACGACTCACTATAGGG-3′) and T7 terminator (5′-GCTAGTTATTGCTCAGCGG-3′). Daptomycin (DAP, Cubicin^®) was generously provided by Merck.