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Human glucagon elisa kit

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The Human Glucagon ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the determination of glucagon levels in human samples. The kit utilizes a specific antibody coated onto the wells of a microplate to capture glucagon present in the samples. The bound glucagon is then detected using a biotinylated detection antibody and an enzyme-labeled streptavidin conjugate.

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Lab products found in correlation

Actin, by Cell Signaling Technology (2 mentions) P s6kt389, by Cell Signaling Technology (2 mentions) Total s6k, by Cell Signaling Technology (2 mentions) Cd41 fitc, by BioLegend (2 mentions) Annexin 5 fitc, by BioLegend (2 mentions) Rat insulin elisa kit, by ALPCO (2 mentions) Pakt t308, by Cell Signaling Technology (2 mentions) Human rcela2a, by MyBioSource (2 mentions) Cela2a elisa kit, by MyBioSource (2 mentions) Cela2a antibody, by Merck Group (2 mentions) N succinyl ala ala pro leu p nitroanilide substrate, by Merck Group (2 mentions) Fitc conjugated pac1 antibody, by BD (2 mentions) Tetramethylrhodamine ethyl ester tmre reagent, by Thermo Fisher Scientific (2 mentions) Total irs, by Cell Signaling Technology (2 mentions) Pirs y608, by Cell Signaling Technology (2 mentions) Pirs s636 s639, by Cell Signaling Technology (2 mentions) A1at antibody ab9400, by Abcam (2 mentions) Itga2b antibodies, by Abcam (2 mentions) Human c peptide elisa kit, by ALPCO (2 mentions) Rat c peptide elisa, by ALPCO (2 mentions)

3 protocols using human glucagon elisa kit

1

Quantitative Platelet and Pancreatic Assays

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Purified GP2B3A complex (MBS135714), mouse rCela2a protein (MBS1246487), human rCELA2A (MBS1090462) and the CELA2A ELISA kit (MBS932150) were purchased from MyBiosource. CELA2A antibody (SAB1104798) and the elastase substrate N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide substrate (S8511) were purchased from Sigma-Aldrich. The FITC-conjugated PAC1 antibody (340507, BD Biosciences) and tetramethylrhodamine ethyl ester (TMRE) reagent (T669, Thermo Fisher Scientific), Annexin V-FITC (640905, Biolegend), CD41-FITC (303703, Biolegend) were used for platelet aggregation analysis. Total AKT (CST, 9272), pAKT (S473) (CST, 9271s), pAKT (T308) (CST, 9275s), total S6k (CST, 9202), pS6k (T389) (CST, 9206), total IRS (CST, 2382), pIRS (Y608), (CST, 2385S), pIRS (S636/S639) (CST, 2388s), Gapdh (CST, 3683s), and Actin (CST, 4970) antibodies were purchased from Cell Signaling Technologies. Recombinant A1AT (alpha1 antitrypsin, ab91136), A1AT antibody (ab9400) and ITGA2B antibodies (ab63983) were purchased from Abcam. Human c-peptide ELISA kit (80-CPTHU-E01.1, ALPCO), Human Glucagon ELISA kit (10-1271-01, Mercodia), Rat c-peptide ELISA (80-CPTRT-E01, ALPCO), Mouse Ultrasensitive Insulin ELISA (80-INSMSU-E01, ALPCO), Human Insulin ELISA kit (21-IAAHU-E01, ALPCO), Rat Insulin ELISA kit (80-INSRT-E01, ALPCO) was applied for C-peptide, glucagon, and insulin measurements.
Esteghamat F., Broughton J.S., Smith E., Cardone R., Tyagi T., Guerra M., Szabó A., Ugwu N., Mani M.V., Azari B., Kayingo G., Chung S., Fathzadeh M., Weiss E., Bender J., Mane S., Lifton R.P., Adeniran A., Nathanson M.H., Gorelick F.S., Hwa J., Sahin-Tóth M., Belfort-DeAguiar R., Kibbey R.G, & Mani A. (2019). CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nature genetics, 51(8), 1233-1243.
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2

Pseudoislet Insulin and Glucagon Secretion

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Batches of 25 pseudoislets were used per donor for in vitro secretion assays in RPMI 1640 (Gibco) supplemented with different levels of glucose. For insulin secretion assays, pseudoislets were pre-incubated for 1 h at 2.8 mM glucose followed by incubation at 2.8, 5.6, 16.7 and 16.7 mM + IBMX glucose concentrations for 60 min each. For glucagon secretion assays, pseudoislets were incubated at 7, 1, 1 mM + 10 mM arginine glucose concentrations for 60 min each. Supernatants were collected, and cells were lysed in acid ethanol to extract insulin and glucagon content. Human insulin in the supernatants and pseudoislet lysates was quantified using a human insulin ELISA kit (Mercodia). Human glucagon in the supernatants and pseudoislet lysates was quantified using a human glucagon ELISA kit (Mercodia). Secreted insulin and glucagon levels are presented as a percentage of gDNA, quantified from the pseudoislet lysates.
Rottner A.K., Ye Y., Navarro-Guerrero E., Rajesh V., Pollner A., Bevacqua R.J., Yang J., Spigelman A.F., Baronio R., Bautista A., Thomsen S.K., Lyon J., Nawaz S., Smith N., Wesolowska-Andersen A., Fox J.E., Sun H., Kim S.K., Ebner D., MacDonald P.E, & Gloyn A.L. (2022). A genome-wide CRISPR screen identifies CALCOCO2 as a regulator of beta cell function influencing type 2 diabetes risk. Nature Genetics, 55(1), 54-65.
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3

Quantitative Platelet and Pancreatic Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified GP2B3A complex (MBS135714), mouse rCela2a protein (MBS1246487), human rCELA2A (MBS1090462) and the CELA2A ELISA kit (MBS932150) were purchased from MyBiosource. CELA2A antibody (SAB1104798) and the elastase substrate N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide substrate (S8511) were purchased from Sigma-Aldrich. The FITC-conjugated PAC1 antibody (340507, BD Biosciences) and tetramethylrhodamine ethyl ester (TMRE) reagent (T669, Thermo Fisher Scientific), Annexin V-FITC (640905, Biolegend), CD41-FITC (303703, Biolegend) were used for platelet aggregation analysis. Total AKT (CST, 9272), pAKT (S473) (CST, 9271s), pAKT (T308) (CST, 9275s), total S6k (CST, 9202), pS6k (T389) (CST, 9206), total IRS (CST, 2382), pIRS (Y608), (CST, 2385S), pIRS (S636/S639) (CST, 2388s), Gapdh (CST, 3683s), and Actin (CST, 4970) antibodies were purchased from Cell Signaling Technologies. Recombinant A1AT (alpha1 antitrypsin, ab91136), A1AT antibody (ab9400) and ITGA2B antibodies (ab63983) were purchased from Abcam. Human c-peptide ELISA kit (80-CPTHU-E01.1, ALPCO), Human Glucagon ELISA kit (10-1271-01, Mercodia), Rat c-peptide ELISA (80-CPTRT-E01, ALPCO), Mouse Ultrasensitive Insulin ELISA (80-INSMSU-E01, ALPCO), Human Insulin ELISA kit (21-IAAHU-E01, ALPCO), Rat Insulin ELISA kit (80-INSRT-E01, ALPCO) was applied for C-peptide, glucagon, and insulin measurements.
Esteghamat F., Broughton J.S., Smith E., Cardone R., Tyagi T., Guerra M., Szabó A., Ugwu N., Mani M.V., Azari B., Kayingo G., Chung S., Fathzadeh M., Weiss E., Bender J., Mane S., Lifton R.P., Adeniran A., Nathanson M.H., Gorelick F.S., Hwa J., Sahin-Tóth M., Belfort-DeAguiar R., Kibbey R.G, & Mani A. (2019). CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nature genetics, 51(8), 1233-1243.
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