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Mvc00

Manufactured by R&D Systems
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Sourced in United States

MVC00 is a piece of lab equipment designed for cell culture applications. It provides controlled temperature and humidity conditions for maintaining cell cultures. The device features precise temperature and humidity monitoring and regulation.

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Lab products found in correlation

Mic100, by R&D Systems (3 mentions) Mmpt90, by R&D Systems (1 mentions) Mmp200, by R&D Systems (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Mg100, by R&D Systems (1 mentions) Mje00, by R&D Systems (1 mentions) Mob00, by R&D Systems (1 mentions)

4 protocols using mvc00

1

Quantification of Plasma Inflammatory Markers in ApoE KO Mice

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Peripheral blood samples of ApoE KO mice were collected from the postcaval vein using a 24-gauge catheter (382412, BD) at 28 days. The blood plasma was separated using Ficoll-Paque plus (#17–1440-03, GE). Circulating IL-6 (M6000B), TGFβ (MB100B), IL-10 (M1000B), tumor necrosis factor (TNF) α (MTA00B), intercellular adhesion molecule (ICAM; MIC100), MMP-9 (MMPT90), vascular cell adhesion molecule (VCAM; MVC00), and MMP-2 (MMP200, all from R&D systems) levels were evaluated using mouse ELISA kits. The ELISA reaction product was quantified by measuring absorbance at 450 nm and 540 nm using a microplate reader (iMark), and data were analyzed using Microplate Manager software (Ver. 6.1, both from Bio-rad).
Kim J.H., Lim I.R., Joo H.J., Park C.Y., Choi S.C., Jeong H.S, & Hong S.J. (2019). Fimasartan reduces neointimal formation and inflammation after carotid arterial injury in apolipoprotein E knockout mice. Molecular Medicine, 25, 33.
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2

Multiplex Biomarker Profiling in Mice

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Serum was aliquoted and frozen at −80°C until analysis. Samples were shipped on dry ice to Dr. Virginia Kraus’s laboratory at Duke University for analysis. Concentrations of IFN-γ, IL-1β, IL-6, IL-10, IL12p70, IL-8 (KC), and TNF-α were measured using the Mouse Pro-inflammatory Ultra-Sensitive sandwich immunoassay 7-plex kit (#K15012C, MSD, USA). Serum concentrations of adiponectin (#K0013182, MSD), IGF-1 (#MG100, R&D, USA), leptin (#MOB00, R&D), CCL2, also known as MCP-1, (#MJE00, R&D), and VCAM-1 (#MVC00, R&D) were measured following manufacturer instructions. Serum dilutions, lowest levels of detection, intra-assay coefficients of variation, and additional details are provided in the Supplemental Methods.
Hahn A.K., Batushansky A., Rawle R.A., Lopes E.B., June R.K, & Griffin T.M. (2021). Effects of Long-Term Exercise and a High-Fat Diet on Synovial Fluid Metabolomics and Joint Structural Phenotypes in Mice: An Integrated Network Analysis. Osteoarthritis and cartilage, 29(11), 1549-1563.
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3

Measuring Adhesion Molecules in Fasted Mice

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In 4-h fasted plasma samples collected as described above from 20-week treated mice (experiment 2), levels of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 were determined by ELISA using the manufacturer's protocols (MIC100 and MVC00, respectively; R&D Systems).
van Eenige R., Ying Z., Tambyrajah L., Pronk A.C., Blomberg N., Giera M., Wang Y., Coskun T., van der Stelt M., Rensen P.C, & Kooijman S. (2021). Cannabinoid type 1 receptor inverse agonism attenuates dyslipidemia and atherosclerosis in APOE∗3-Leiden.CETP mice. Journal of Lipid Research, 62, 100070.
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4

Plasma ICAM-1 and VCAM-1 Quantification

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Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were determined in plasma samples obtained after 10 weeks of treatment (experiment 1) by ELISA using manufacturer's protocols (MIC100 and MVC00, respectively; R&D Systems).
van Eenige R., Ying Z., Tramper N., Wiebing V., Siraj Z., de Boer J.F., Lambooij J.M., Guigas B., Qu H., Coskun T., Boon M.R., Rensen P.C.N, & Kooijman S. (2023). Combined glucose-dependent insulinotropic polypeptide receptor and glucagon-like peptide-1 receptor agonism attenuates atherosclerosis severity in APOE*3-Leiden.CETP mice. Atherosclerosis, 372.
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