Agilent 19091s 433ui

Analysis used an Agilent 7890B gas chromatography instrument, combined with an Agilent-5977A mass spectrometer (Agilent Technologies) equipped with electron ionization (EI) and quadrupole analyzer, and an Agilent Chem Station data system. GC separation was performed on a 30 m HP-5ms Ultra Inert capillary column with an internal diameter of 0.25 mm and a film thickness of 0.25 μm (Agilent 19091S-433UI, Agilent Technologies).

GC–MS analysis for identification of isoeugenol in the fruits of S. chinensis was performed using an Agilent Technologies 7890B gas chromatography instrument, combined with an Agilent 5977A MSD equipped with electron ionization (EI) and quadrupole analyzer, and an Agilent Chem Station data system. GC separation was performed on a 30 m HP-5MS Ultra Inert capillary column with an internal diameter of 0.25 mm and a film thickness of 0.25 μm (Agilent 19091S-433UI, Agilent Technologies). The carrier gas was helium (99.99%) with a flow rate of 1 ml/min. The temperature of the injector and detector was set at 280°C and 250°C, respectively. Spectra were obtained over a scan range of 50 to 550 amu at 2.9 scans/s. The GC program was set as follows: the initial temperature was 60°C and held for 2 min, then increased by 5°C/min to 135°C, then raised by 3°C/min to 180°C, and finally raised by 10°C/min to 200°C and held at 200°C for 10 min. The sample and isoeugenol solution (1 μl) were injected manually while maintaining a solvent delay of 4 min. Interpretation of the mass spectrum was made by comparing the peak distribution against the database of National Institute Standard and Technology (NIST MS 2.0, Gaithersburg, MD, USA).

The equipment used for the study are Centrifuge (800D) from Gulfex medical and scientific, England; Spectrophotometer from Spectrum Lab and 52b New life medical instrument, England; Water Bath (OLS200) from Grant Instrument (Cambridge) LTD; Weighing Balance (Scout Pro Spo402) from Ohaus Corporation, Pine Brrok, NJ, USA; FTIR (Cary630) from Agilent Technology, Malaysia; Heater (HP139920-33) from Barnstead Thermolyne, England; Glucometer (CE0088) from Roche Diagnostic GmnH, Germany; and GC-MS from Agilent 19091S-433UI Agilent Technologies, United States.