Left lungs were macerated and filtered through a nylon mesh (70 µm; Spectrum Labs, Rancho Dominguez, CA, USA) in 2 mL PBS and centrifuged at 1,500 rpm at 4°C for 10 min. Supernatants were collected, and the IL-4, IL-5, and IL-10 levels were evaluated using the kit Mouse OptEIA (BD PharMingen) and IL-13 levels using the Mouse DuoSet ELISA (R&D Systems Inc., McKinley, MN, USA) according to the manufacturer’s instructions. The concentration of the cytokines was determined through standard curves generated using different concentrations of recombinant cytokines. The limit of detection for each cytokine was as follows: IL-4: 7.8 pg/mL, IL-5: 15.6 pg/mL, IL-13: 62.5 pg/mL, and IL-10: 31.3 pg/mL.
Mouse opteia
Manufactured by BD
Sourced in United States, Canada
The Mouse OptEIA is a laboratory equipment product designed for enzyme-linked immunosorbent assay (ELISA) testing. It provides the necessary components to perform quantitative measurements of mouse-specific analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse opteia
1
Cytokine Levels in Lung Macerate
2
Splenocyte Cytokine Secretion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Splenocytes were used to determine cytokine secretion profiles following antigen re-stimulation as previously described (29) . Briefly, splenocyte suspensions were plated onto 96-well U-bottom tissue culture plates in triplicate (100 μl per well) along with 100 μl of OVA such that the final concentration was 0.5 mg ml -1 . Cells were incubated for 24 h (for IL-2) and 72 h (for IFN-γ). Commercially available assay kits (mouse OptEIA; BD Biosciences, Mississauga, Ontario, Canada) were used according to the manufacturer's instructions to assay cytokine levels in culture supernatants.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!