All enzymes, as well as plasmid pGEM1, TNT T7 Quick Coupled System and rabbit reticulocyte lysate were from Promega (Madison, WI, USA). ER rough microsomes from dog pancreas were from tRNA Probes (College Station, TX, USA). EasyTag™ EXPRESS35S Protein Labeling Mix, [35S]-L-methionine and 35S-L-cysteine, for in vitro labeling was purchased from Perkin Elmer (Waltham, MA, USA). Restriction enzymes and Endoglycosidase H were from Roche Molecular Biochemicals (Basel, Switerland). Proteinase K was from Sigma-Aldrich (St Louis, MO). The DNA plasmid, RNA clean-up and PCR purification kits were from Thermo Fisher Scientific (Ulm, Germany). All oligonucleotides were purchased from Macrogen (Seoul, South Korea).
Plasmid pgem1
Manufactured by Promega
Sourced in United States
Plasmid pGEM1 is a commonly used cloning vector. It contains a multiple cloning site and allows for the insertion and propagation of DNA fragments.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit reticulocyte lysate, by Promega (2 mentions)
Restriction enzymes, by Roche (2 mentions)
Endoglycosidase h, by Roche (2 mentions)
Pcr purification kit, by Thermo Fisher Scientific (2 mentions)
Dna plasmid, by Thermo Fisher Scientific (2 mentions)
Rna clean up, by Thermo Fisher Scientific (2 mentions)
Proteinase k, by Merck Group (2 mentions)
Tnt t7 quick coupled system, by Promega (1 mentions)
Easytag express35s protein labeling mix, by PerkinElmer (1 mentions)
2 protocols using plasmid pgem1
1
In Vitro Protein Synthesis and Labeling
2
In Vitro Protein Translation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All enzymes, as well as plasmid pGEM1, RiboMAX SP6 RNA polymerase system and rabbit reticulocyte lysate were from Promega (Madison, WI). ER rough microsomes from dog pancreas were from tRNA Probes (College Station, TX, USA). [ 35 S]Met and [ 14 C]-methylated markers were from Perkin Elmer. Restriction enzymes and Endoglycosidase H were from Roche Molecular Biochemicals. Proteinase K was from Sigma-Aldrich (St Louis, MO). The DNA plasmid, RNA clean up and PCR purification kits were from Thermo Fisher Scientific (Ulm, Germany). All oligonucleotides were purchased from IDT (USA).
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