293 T and 293FT cells (R700-07) were purchased from Invitrogen, and Jurkat T cells were purchased from ATCC. The medium used for these cell lines was Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum. Transfections were carried out using Lipofectamine 2000 (Invitrogen), except for Jurkat T cells, which underwent electroporation with the Amaxa kit according to the manufacturer’s instructions. The luciferase assay was performed using the luciferase reporter gene assay kit (Promega) according to the manufacturer’s instructions. One day before transfection, 5 × 105 cells were seeded in 6-well culture plates, and the assay was performed after 30 h of transfection.
293 t and 293ft cells
Manufactured by Thermo Fisher Scientific
The 293 T and 293FT cells are widely used human embryonic kidney cell lines. They are derived from the original 293 cell line and are commonly used in cell and molecular biology research as well as in the production of viral vectors and recombinant proteins.
Automatically generated - may contain errors
Lab products found in correlation
Luciferase reporter gene assay kit, by Promega (1 mentions)
Jurkat t cells, by American Type Culture Collection (1 mentions)
Jurkat t cells, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Nacalai Tesque (1 mentions)
Ham s f 10 medium, by Fujifilm (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Non essential amino acid solution, by Nacalai Tesque (1 mentions)
Hek blue tgf β cells, by InvivoGen (1 mentions)
Fetal bovine serum (fbs), by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
2 protocols using 293 t and 293ft cells
1
Transfection and Luciferase Assay in Cell Lines
2
Cell Culture of Melanoma and Kidney Cell Lines
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The B16-F0 mouse melanoma cell line (cat. no. JCRB0202) was purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank and maintained in Eagle's Minimum Essential Medium (EMEM; FUJIFILM Wako Pure Chemical Corporation) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich; Merck KGaA), 100 U/ml penicillin and 100 µg/ml streptomycin (both from Nacalai Tesque, Inc.) under mycoplasma-free conditions. Clone M3 (Cloudman S91) melanoma cell line was obtained from European Collection of Authenticated Cell Cultures (ECACC) and cultured in Ham's F10 medium (FUJIFILM Wako Pure Chemical Corporation) supplemented with 15% FBS, 2 mM glutamine (Nacalai Tesque, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin. 293T and 293FT cells were obtained from Invitrogen; Thermo Fisher Scientific, Inc. HEK-Blue™ TGF-β cells were purchased from InvivoGen. 293T, 293FT and HEK-Blue™ TGF-β cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Inc.) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. The cultured medium for 293FT cells was also supplemented with 1% non-essential amino acid solution (Nacalai Tesque, Inc.). All cell lines were cultured in a humidified incubator containing 5% CO2 at 37°C.
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