All chemicals, unless otherwise stated, were of the highest quality and were used as supplied. Carvacrol (99.9%), thymol (98.5%), dichlorofluorescin diacetate (DCFH-DA), N-acetyl-l -cysteine (NAC), and trypan blue were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640 culture medium with l -glutamine, fetal bovine serum (FBS), penicillin–streptomycin, Hank’s Buffered Saline Solution (HBSS), and phosphate-buffered Saline EDTA (PBS) were purchased from Lonza (Basel, Switzerland). Dimethylsulfoxide (DMSO) was obtained from Molecular Probes (Eugene, OR, USA). Annexin-V-FITC, propidium iodide (PI), and Z-VAD-FMK were obtained from BD Pharmingen (San Diego, CA, USA). Caspase-3, caspase-9, Chop, p-AKT, PI3K, and β-actin antibodies were obtained from Cell Signaling (Danvers, MA, USA).
Hank s buffered saline solution hbss
Manufactured by Lonza
Sourced in Switzerland
Hank's Buffered Saline Solution (HBSS) is a balanced salt solution used in cell culture and other laboratory applications. It maintains the pH and osmotic balance of cells and tissues in vitro. HBSS contains a blend of inorganic salts, glucose, and phenol red as a pH indicator.
Automatically generated - may contain errors
Lab products found in correlation
Trypan blue, by Merck Group (1 mentions)
Z vad fmk, by BD (1 mentions)
Thymol, by Merck Group (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Carvacrol, by Merck Group (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Propidium iodide, by BD (1 mentions)
Napyruvate, by Corning (1 mentions)
Phenol red free low glucose dmem, by Merck Group (1 mentions)
Gsh gssg glo assay, by Promega (1 mentions)
2 protocols using hank s buffered saline solution hbss
1
Carvacrol and Thymol Cytotoxicity Assay
2
Rat Alveolar Cell Culture Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Materials were obtained from the following suppliers: female rat non-cancerous type-II alveolar cells (CC-149, L2 cells) and fetal bovine serum (FBS), ATCC; low glucose DMEM, Hyclone. Phenol red-free low glucose DMEM, 17β-estradiol (E2) and 3,3′, 5-triiodo-L-thyronine sodium salt (T3), Sigma-Aldrich; charcoal-stripped FBS and 100X antibiotic-antimycotic, Invitrogen; Trypsin-EDTA .05%, VWR; Na-pyruvate, Cellgro; Hanks buffered saline solution (HBSS), Lonza; MTT, PGE2, and Apotox-Glo Triplex assay kits from Roche, Cayman Chemical, and Promega, respectively. Promega generously provided GSH/GSSG-Glo assay prior to public release.
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