Absolute ethanol

Trolox^® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonic acid)), DTT (DL-Dithiothreitol), Trizma^® base, fluorescein sodium salt, diatomaceous earth (Hyflo^® Super Cel^®), and formic acid (reagent grade ≥ 95%) were provided by Sigma-Aldrich (Steinheim, Germany). Potassium dihydrogen phosphate, sodium phosphate dibasic, potassium sulfate, sodium chloride, ortho-boric acid, TRIS (ultrapure), AAPH (2,2′-azobis (2-amidinopropane)) (Acros Organics), glycine (proteomics grade), and methanol (HPLC LC-MS grade) were purchased from VWR International Eurolab S.L. (Barcelona, Spain). Sodium hydroxide, glacial acetic acid, and sulfuric acid were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate, purissimum-CODEX) was obtained from Panreac (Barcelona, Spain). Acetonitrile (HPLC grade), acetone, glycerol, and bromophenol blue indicator (ACS reagent) were supplied by Merck (Darmstadt, Germany). Anhydrous magnesium sulfate (99.5% min powder) was provided by Alfa Aesar (Karlsruhe, Germany). Octadecyl C18 sorbent was from Phenomenex (Madrid, Spain) while absolute ethanol was from J.T. Baker (Deventer, The Netherlands). Deionized water (resistivity >18 MΩ cm⁻¹) was obtained through a Milli-Q SP^® Reagent Water System (Millipore Corporation, Bedford, MA, USA).

ABTS (2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid)), Trolox^® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DTT (dl-dithiothreitol), diatomaceous earth (Hyflo^® Super Cel^®), Trizma^® base, fluorescein sodium salt, and formic acid (reagent grade ≥ 95%) were purchased from Sigma-Aldrich (Steinheim, Germany). AAPH (2,2′-azobis(2-amidinopropane)) (Acros Organics), Tris (ultrapure), potassium dihydrogen phosphate, potassium sulfate, sodium phosphate dibasic, sodium chloride, ortho-boric acid, glycine (proteomics grade), and methanol (HPLC–MS grade) were provided by VWR International Eurolab S.L. (Barcelona, Spain). Glacial acetic acid, sulfuric acid, and sodium hydroxide were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate, purissimum-CODEX) and nitric acid (65% p/p) were obtained from Panreac (Barcelona, Spain). Acetonitrile (HPLC grade), acetone, glycerol, and bromophenol blue indicator (ACS reagent) were supplied by Merck (Darmstadt, Germany). Octadecyl C18 sorbent was from Phenomenex (Madrid, Spain), while absolute ethanol was from J.T. Baker (Deventer, The Netherlands). Anhydrous magnesium sulfate (99.5% min powder) was provided by Alfa Aesar (Karlsruhe, Germany). Deionized water (resistivity >18 MΩ/cm) was obtained through a Milli-Q SP^® Reagent Water System (Millipore Corporation Bedford, Bedford, MA, USA).