DNA was isolated and labelled using Agilent-recommended protocol. CGH was performed on an Agilent Microarray platform with 105k probes (Agilent Inc., Santa Clara, CA). Array image was acquired with an Agilent Array Scanner. Microarray data was analyzed using the Cytogenomics software package from Agilent Inc. Interpretation of detected CNVs was conducted according to the ACMG standards and guidelines revision 2013 [13 (link)], and technical standards recommendation by ACMG and ClinGen [14 (link)]. Information for clinical significance on reported cases was extracted from Databases of Genomic Variants (ClinVar) at www.ncbi.nim.nih.gov/clinvar , and DECIPHER at www.decipher.sanger.ac.uk . Association analysis of gene function and clinical features is based on the information from Online Mendelian Inheritance in Man (OMIM at www.omim.org ).
Agilent microarray platform with 105k probes
Manufactured by Agilent Technologies
The Agilent Microarray platform with 105k probes is a high-density microarray designed for comprehensive genome analysis. The platform features 105,000 distinct probes, enabling researchers to evaluate a wide range of genomic targets in a single experiment.
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2 protocols using agilent microarray platform with 105k probes
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Comprehensive Genomic Profiling Using CGH
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Comprehensive Genomic Profiling Using CGH
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DNA was isolated and labelled using Agilent-recommended protocol. CGH was performed on an Agilent Microarray platform with 105k probes (Agilent Inc., Santa Clara, CA). Array image was acquired with an Agilent Array Scanner. Microarray data was analyzed using the Cytogenomics software package from Agilent Inc. Interpretation of detected CNVs was conducted according to the ACMG standards and guidelines revision 2013 [13 (link)], and technical standards recommendation by ACMG and ClinGen [14 (link)]. Information for clinical significance on reported cases was extracted from Databases of Genomic Variants (ClinVar) at www.ncbi.nim.nih.gov/clinvar , and DECIPHER at www.decipher.sanger.ac.uk . Association analysis of gene function and clinical features is based on the information from Online Mendelian Inheritance in Man (OMIM at www.omim.org ).
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