Pe anti mouse cd3 monoclonal antibody mab

Human primary CD3⁺ T cells were isolated from healthy people’s blood using a CD3⁺ Microbead kit (Miltenyi Biotec Inc. CA, USA), according to the manufacturer’s instructions. The purity of the CD3⁺ T-cell preparation was assessed by flow cytometry (FACS Calibur, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) using PE-anti mouse CD3 monoclonal antibody (mAb) (eBioscience, Inc., San Diego, CA, USA).
Jurkat cells (human T cell line), LNCaP and PC-3 (human prostate cancer cell lines), were cultured in RPMI-1640 (GE Healthcare Life Sciences Hyclone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences Hyclone Laboratories), 10 µg/mL penicillin and 10 µg/mL streptomycin (Beijing Solarbio Science & Technology Co., Ltd. Beijing, China). The cell culture was maintained in an incubator at 37 °C with 5% CO₂.

Human primary CD3⁺T cells were isolated from healthy people blood using a CD3εMicroBead kit (Miltenyi Biotec Inc. CA, USA), according to the manufacturer’s instructions. The purity of the CD3⁺ T-cell preparation was assessed by flow cytometry (FACS Calibur, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) using PE-anti-mouse CD3monoclonal antibody (mAb) (eBioscience, Inc., San Diego, CA, USA).
Jurkat cells (human T cell line), HK-2 (human renal tubular epithelial cell line), LNCaP and PC-3 (human prostate cancer cell lines), EL-4 (murine T cell line), and RM-1 (murine prostate cancer cell line), TCMK-1 (murine renal tubular epithelial cell line) were cultured in RPMI1640 (GE Healthcare Life Sciences Hyclone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences Hyclone Laboratores), 10 μg/ml penicillin and 10 μg/ml streptomycin (Beijing Solarbio Science & Technology Co., Ltd. Beijng, China). RWPE-1 cells were cultured in serum free SFM media. The cell culture was maintained in an incubator at 37 °C with 5% CO₂.