The following antisera were used: α-pMAD (from Ed Laufer and P. ten Dijke, at 1:2000; 51); α-dpERK (Sigma; 1:250), α-Apontic (from R. Schuh,; 52); α-Discs large (4F3; 1:50), α-DE-cadherin (DECAD2; 1:20), and anti-β-galactosidase were from Developmental Studies Hybridoma Bank. dpERK staining was carried out as described (9 ) with antibody obtained from Cell Signaling. Secondary antibodies were conjugated to Alexa-Fluor 488, 555 or 647. To assay for cell lethality, α-cleaved caspase-3 (Asp175, Cell Signaling) was used as described (53 (link)). Cell proliferation was monitored with α-phosphohistone H3 antibody (Ser 10, Cell Signaling).
Anti β galactosidase
Manufactured by Developmental Studies Hybridoma Bank
Anti-β-galactosidase is an antibody product available from the Developmental Studies Hybridoma Bank. It is a laboratory tool used for the detection and quantification of the enzyme β-galactosidase.
Automatically generated - may contain errors
2 protocols using anti β galactosidase
1
Immunohistochemical Analysis of Developmental Markers
2
Antibody Panel for Developmental Studies
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