Endocervical sampling was conducted as described previously elsewhere (11 (link)). Briefly, the cervical specimens were collected with a ThinPrep kit (Hologic) using a cytobrush. The cytobrush was rinsed into 20 mL of PreservCyt (Hologic) fixative solution, which was acidified to dissolve mucous and centrifuged at 400 × g for 5 minutes at 4°C. After washing the cells three times in 20 mL of phosphate buffered saline (PBS), the cells were brought to 10 mL with PBS at 4°C. An aliquot (~1 mL) of each processed specimen containing up to 200,000 cells was centrifuged onto a slide using a Shandon Cytospin 3 centrifuge (Thermo-Fisher) and labeled with 10 μg/mL of mouse anti-HLA-G (Clone 4H84, BD Biosciences; or Clone G233, Exbio) to estimate the content of trophoblast cells, as previously described elsewhere (11 (link)).
Clone g233
Manufactured by Exbio
The Clone G233 is a laboratory instrument designed for cell culture applications. It functions as a semi-automated cell culture system capable of maintaining and expanding a variety of cell lines.
Automatically generated - may contain errors
2 protocols using clone g233
1
Endocervical Sampling for Trophoblast Cell Analysis
2
Extraction and Identification of Trophoblast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Endocervical specimens were obtained, as previously described23 (link), using a ThinPrep kit (Hologic, Inc., Marlborough, MA) with cytobrush after placing a speculum in the vagina. Cervical mucus was included with the specimens. The cytobrush was rinsed into 20 ml of PreservCyt (Hologic) fixative solution and transported to the laboratory. Mucus was dissolved by adding 500 μl concentrated glacial acetic acid for 5 min to each specimen. Specimens were centrifuged at 400 × g for 5 min at 4 °C, the supernatant was removed, and the cell pellet was resuspended in 20 ml of ice-cold sterile phosphate buffered saline (PBS). The washing procedure was repeated twice and the final pellet was resuspended in 10 ml of PBS. To determine the content of trophoblast cells, a small aliquot of each processed specimen was centrifuged onto a slide using a Shandon Cytospin 3 centrifuge (Thermo-Fisher, Waltham, MA) at 1500 RPM for 5 min and labeled with 10 μg/ml of mouse anti-HLA-G (Clone 4H84, BD Biosciences, San Diego, CA or Clone G233, Exbio, Prague, Czech Republic), as previously described23 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!