Tetramethylrhodamine β isothiocyanate tritc or fluorescein isothiocyanate fitc conjugated lycopersicon esculentum agglutinin tomato lectin

Human Fg (FIB-3, depleted of plasminogen, von-Willebrand factor, and fibronectin) was purchased from Enzyme Research Laboratories (South Bend, IN). Rat anti-mouse PV-1 monoclonal antibody (clone: MECA-32; Isotype: IgG2a) was from AbD Serotec (Raleigh, NC). Polyclonal antibody against Cav-1 was obtained from Novus Biological (Littleton, CO). Purified mouse anti phospho-Cav-1 (pCav-1; pY14) and A431- Epidermal growth factor (A431-EGF) cell lysate were purchased from BD Biosciences (San Diego, CA). 4,6-diamidino-2-phenyl-indole HCl (DAPI) was from Santa Cruz Biotechnology (Santa Cruz, CA). Secondary antibodies conjugated with Alexa-Fluor 488 (chicken anti-rat IgG), or with Alexa-Fluor 594 (chicken anti-rat IgG and rabbit anti-goat IgG) were purchased from Invitrogen (Carlsbad, CA). Bovine serum albumin (BSA) was from Sigma Aldrich Chemicals Co. (St. Louis, MO). Normal Donkey Serum (NDS) was obtained from Jackson ImmunoResearch (West Grove, PA). Tetramethylrhodamine β-isothiocyanate (TRITC)- or fluorescein isothiocyanate (FITC)-conjugated Lycopersicon esculentum agglutinin (LEA) tomato lectin was from Vector laboratories (Burlingame, CA). Artificial cerebrospinal fluid (CSF, composition: Na 150 mM Na; 3.0 mM K; 1.4 mM Ca; 0.8 mM Mg; 1.0 mM P; 155 mM Cl) was purchased from Harvard Apparatus (Holliston, MA). RIPA buffer was from Boston BioProducts (Worcester, MA)

Polyclonal rabbit anti-human Fg antibody was from Dako (Carpinteria, CA). Purified antibody against mouse ICAM-1 (CD54 - clone: YN1/1.7.4; Isotype: Rat IgG2b,κ) was obtained from BioLegend (San Diego, CA). Purified mouse anti phospho-caveolin-1 (pY14) was purchased from BD Biosciences (San Diego, CA). Rat anti-mouse PV-1 monoclonal antibody (clone: MECA-32; Isotype: IgG2a) was from AbD Serotec (Raleigh, NC). Polyclonal antibody against Cav-1 was obtained from Novus Biological (Littleton, CO). DQ gelatin, 1,10-phenanthroline,monohydrate, and secondary antibodies conjugated with Alexa-Fluor 488, Alexa-Fluor 594, or Alexa-Fluor 647 were purchased from Invitrogen (Carlsbad, CA). Monoclonal anti-Prion protein was from Sigma Aldrich Chemicals Co. (St. Louis, MO). 4,6-diamidino-2-phenyl-indole HCl (DAPI) were from Santa Cruz Biotechnology (Santa Cruz, CA). Normal Donkey Serum (NDS) was purchased from Jackson ImmunoResearch (West Grove, PA). Tetramethylrhodamine β-isothiocyanate (TRITC)- or fluorescein isothiocyanate (FITC)-conjugated Lycopersicon esculentum agglutinin (LEA) tomato lectin was from Vector laboratories (Burlingame, CA). Artificial cerebrospinal fluid (CSF) was purchased from Harvard Apparatus (Holliston, MA).