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4 protocols using rabbit anti ha tag antibody

1

Antibody Procurement for Protein Detection

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Rabbit anti-ADAM9, anti-Flag tag, and mouse anti-Flag tag antibodies were purchased from Cell Signaling Technology (2099s, 2368s, and 8146s). Mouse anti-HIV p24 antibody was purchased from Abcam (ab9071). Rabbit anti-GAPDH antibody was purchased from GeneTex (GTX100118). Rabbit anti-HA tag antibody was purchased from Millipore (04–902). Normal rabbit IgG was purchased from Santa Cruz (sc-2027). Goat anti-ACE2 antibody was purchased from R&D Systems (AF933). All Alexa Fluor-conjugated secondary antibodies used for immunofluorescence were procured from Molecular Probes (Invitrogen). NH4Cl, BafA1, and poly-L-lysine solution (P4707) were purchased from Sigma-Aldrich. Batimastat (BB-94) was purchased from Calbiochem (196440).
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2

Antibody Procurement for Protein Detection

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Rabbit anti-ADAM9, anti-Flag tag, and mouse anti-Flag tag antibodies were purchased from Cell Signaling Technology (2099s, 2368s, and 8146s). Mouse anti-HIV p24 antibody was purchased from Abcam (ab9071). Rabbit anti-GAPDH antibody was purchased from GeneTex (GTX100118). Rabbit anti-HA tag antibody was purchased from Millipore (04–902). Normal rabbit IgG was purchased from Santa Cruz (sc-2027). Goat anti-ACE2 antibody was purchased from R&D Systems (AF933). All Alexa Fluor-conjugated secondary antibodies used for immunofluorescence were procured from Molecular Probes (Invitrogen). NH4Cl, BafA1, and poly-L-lysine solution (P4707) were purchased from Sigma-Aldrich. Batimastat (BB-94) was purchased from Calbiochem (196440).
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3

Wnt3a Signaling Pathway Modulation

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Rabbit anti-Wnt3a and anti-Flag tag antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA (2721s, and 2368s). Rabbit anti-GAPDH and goat anti-rabbit IgG antibodies were purchased from GeneTex (GTX100118 and GTX213110-01). Mouse anti-β-catenin was procured from BD Biosciences (BD610154). Rabbit anti-HA tag antibody was purchased from Millipore (04-902). Normal rabbit IgG was purchased from SantaCruz (sc-2027). Lipofectamine™ 2000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA) and TransIT®-LT1 Transfection Reagent (Mirus Bio, Madison, WI, USA) were used for plasmid transfection of H1650 and HEK293T cells, respectively. iCRT-14 and LGK-974 were purchased from MedChemExpress, Monmouth Junction, NJ, USA. The control and Wnt3a-conditioned media (CM) were prepared as previously described [35 (link)].
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4

SARS-CoV-2 and Influenza Protein Detection

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For Western blotting, rabbit anti-ACE2, rabbit anti-IAV nucleoprotein (NP), and goat anti-rabbit IgG antibodies were purchased from GeneTex (GTX101395, GTX629633, and GTX213110-01). Rabbit anti-HA tag antibody was purchased from Millipore (04-902). Mouse anti-IAV matrix protein (M1) antibody was purchased from AbD Serotec (MCA401). For immunofluorescence staining, a mouse anti-IAV NP antibody was purchased from Abcam (ab20343). Alexa Fluor-conjugated secondary antibodies used for immunofluorescence were procured from Molecular Probes (Invitrogen). DAPI was purchased from Sigma-Aldrich.
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