Rabbit polyclonal antibody against gfp

N-hexanoyl-d-erythro-sphingosine (d-cer-C6) and N-hexanoyl-l-erythro-sphingosine (l-cer-C6) obtained from Matreya were dissolved in pure ethanol (Merck) as stock solution. Cycloheximide was purchased from A.G. Scientific, rapamycin was obtained from EMD Millipore, and both were dissolved in DMSO as stock solutions. Sheep anti–human TGN46 was obtained from AbD Serotec. Goat anti-GRASP65 (C-20) was obtained from Santa Cruz Biotechnology, Inc. Mouse anti–β-actin (clone AC-15) was obtained from Sigma-Aldrich. Rabbit polyclonal antibody against GFP was purchased from Abcam. Mouse anti-p230, mouse anti-Sec31a, and mouse anti-EEA1 were obtained from BD. Mouse anti-M6PR (mannose-6 phosphate receptor) was obtained from Thermo Fisher Scientific, mouse anti–transferrin receptor was purchased from Invitrogen, and mouse anti-LAMP1 was obtained from Stressgen. Alexa Fluor–labeled secondary antibodies were obtained from Invitrogen, and HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. Protein A–gold was obtained from the Department of Cell Biology at Utrecht University (Utrecht, Netherlands).

Mouse monoclonal and rabbit polyclonal antibodies against FLAG (Sigma), HA (Sigma), mouse monoclonal antibodies against β-actin (Sigma), NS3 (Abcam) and rabbit polyclonal antibody against GFP (Abcam), 25-hydroxycholesterol (Sigma) were purchased from the indicated companies.